Developing predictive assays: the phenotypic screening "rule of 3"
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Published on: May 29, 2020
Fabien Vincent1, Paula Loria2, Marko Pregel3
1Hit Discovery and Lead Profiling, Pfizer, Groton, CT 06340, USA. fabien.vincent@pfizer.com.
Abstract:
Phenotypic drug discovery approaches can positively affect the translation of preclinical findings to patients. However, not all phenotypic assays are created equal. A critical question then follows: What are the characteristics of the optimal assays? We analyze this question and propose three specific criteria related to the disease relevance of the assay-system, stimulus, and end point-to help design the most predictive phenotypic assays.
