Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genetic Screens02:46

Genetic Screens

5.9K
Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
5.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clickable Substrate Transport (CST): A High-Throughput Functional Assay for Solute Carrier Proteins.

ACS chemical biology·2026
Same author

Comparative study of absorption prediction using human intestinal organoid-derived cells in static and flow conditions.

Drug metabolism and disposition: the biological fate of chemicals·2026
Same author

Hypertension With High-Risk Features in Cryptogenic Stroke: An Exploratory Analysis of the ARCADIA Randomized Clinical Trial.

JAMA neurology·2026
Same author

High throughput, automated and fully contained cytokine release assay using primary human CD4<sup>+</sup> T cells.

SLAS discovery : advancing life sciences R & D·2026
Same author

Immediate Psychological Responses to Aerobic and Resistance Exercise in People With Psychotic Disorders: A Randomized Controlled Trial in Psychiatric Rehabilitation.

Schizophrenia bulletin·2026
Same author

Role of exercise physiologists in mental health support and recovery: 2026 Exercise and Sports Science Australia (ESSA) Consensus Statement.

British journal of sports medicine·2026

Related Experiment Video

Updated: Apr 8, 2026

A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases
06:50

A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases

Published on: May 29, 2020

3.2K

Developing predictive assays: the phenotypic screening "rule of 3".

Fabien Vincent1, Paula Loria2, Marko Pregel3

  • 1Hit Discovery and Lead Profiling, Pfizer, Groton, CT 06340, USA. fabien.vincent@pfizer.com.

Science Translational Medicine
|June 26, 2015
PubMed
Summary

Designing optimal phenotypic assays is key for drug discovery. This study proposes three criteria—disease relevance of the system, stimulus, and endpoint—to create more predictive assays for better translation to patients.

More Related Videos

A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19
06:46

A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19

Published on: July 5, 2022

3.4K
High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
09:44

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

Published on: March 3, 2015

10.1K

Related Experiment Videos

Last Updated: Apr 8, 2026

A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases
06:50

A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases

Published on: May 29, 2020

3.2K
A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19
06:46

A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19

Published on: July 5, 2022

3.4K
High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
09:44

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

Published on: March 3, 2015

10.1K

Area of Science:

  • Drug discovery and development
  • Translational medicine
  • Assay development

Background:

  • Phenotypic drug discovery (PDD) shows promise for translating preclinical findings to clinical applications.
  • However, the predictive power of PDD assays varies significantly.
  • Optimizing assay design is crucial for successful drug development.

Purpose of the Study:

  • To identify the key characteristics of optimal phenotypic assays.
  • To provide criteria for designing more predictive PDD assays.
  • To improve the translation of preclinical research to patient benefit.

Main Methods:

  • Analysis of existing phenotypic assay design principles.
  • Proposal of three core criteria for assay optimization.
  • Focus on disease relevance across assay components.

Main Results:

  • Identified three critical criteria for designing predictive phenotypic assays.
  • These criteria relate to the disease relevance of the assay system, stimulus, and endpoint.
  • The proposed framework aims to enhance assay reliability.

Conclusions:

  • Phenotypic assays must be carefully designed to maximize their predictive value.
  • Adherence to the proposed criteria can lead to more effective drug discovery pipelines.
  • Optimized assays are essential for bridging the gap between preclinical research and patient outcomes.