Ionic liquid-based zwitterionic organic polymer monolithic column for capillary hydrophilic interaction...

Area of Science:

• Analytical Chemistry
• Chromatography Science

Background:

• Hydrophilic interaction chromatography (HILIC) is crucial for separating polar compounds.
• Development of novel stationary phases is essential for improving chromatographic performance.

Purpose of the Study:

• To develop a novel ionic liquid-based zwitterionic organic polymer monolithic column for capillary HILIC.
• To optimize the monolithic column and evaluate its separation efficiency for nucleosides and benzoic acid derivatives.

Main Methods:

• Copolymerization of 1-vinyl-3-(butyl-4-sulfonate) imidazolium, acrylamide, and N,N'-methylenebisacrylamide.
• Optimization of monomer concentration and porogenic solvent composition (formamide, DMSO, PEG 8000, PEG 10,000).
• Evaluation of mobile phase parameters (acetonitrile content, pH, buffer salt concentration).

Main Results:

• The optimized monolith demonstrated excellent selectivity and favorable retention for nucleosides and benzoic acid derivatives.
• High reproducibility was achieved for nucleoside retention times (RSDs < 6.50% for batch-to-batch).
• The column effectively separated target analytes under optimized conditions.

Conclusions:

• The developed ionic liquid-based zwitterionic monolithic column is a promising stationary phase for capillary HILIC.
• The column offers excellent separation performance and reproducibility for polar compounds.
• This novel material advances HILIC applications in analytical chemistry.