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Updated: Apr 8, 2026

Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques
Published on: July 5, 2018
Solution Structure of CCL19 and Identification of Overlapping CCR7 and PSGL-1 Binding Sites
Christopher T Veldkamp1,2, Eva Kiermaier3, Skylar J Gabel-Eissens1
1†Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.
Abstract:
CCL19 and CCL21 are chemokines involved in the trafficking of immune cells, particularly within the lymphatic system, through activation of CCR7. Concurrent expression of PSGL-1 and CCR7 in naive T-cells enhances recruitment of these cells to secondary lymphoid organs by CCL19 and CCL21. Here the solution structure of CCL19 is reported. It contains a canonical chemokine domain. Chemical shift mapping shows the N-termini of PSGL-1 and CCR7 have overlapping binding sites for CCL19 and binding is competitive. Implications for the mechanism of PSGL-1's enhancement of resting T-cell recruitment are discussed.
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