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Related Concept Videos

Immunocytochemistry and Immunohistochemistry01:22

Immunocytochemistry and Immunohistochemistry

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Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
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Related Experiment Video

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A Next-generation Tissue Microarray ngTMA Protocol for Biomarker Studies
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Validity of Tissue Microarray by Immunohistochemistry.

Hiromi Fujita, Tatsuya Takayama, Naohisa Takaoka

    Clinical Laboratory
    |June 30, 2015
    PubMed
    Summary
    This summary is machine-generated.

    Tissue microarrays (TMAs) effectively analyze renal cell carcinoma (RCC) specimens using FABP7 and Brn2 antibodies. This method showed high agreement with traditional methods, regardless of tumor size, for accurate RCC analysis.

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    Production of Tissue Microarrays, Immunohistochemistry Staining and Digitalization Within the Human Protein Atlas
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    Area of Science:

    • Oncology
    • Pathology
    • Biotechnology

    Background:

    • Tissue microarrays (TMAs) enable the analysis of multiple tissue specimens simultaneously.
    • Renal cell carcinoma (RCC) is a significant health concern requiring accurate diagnostic methods.
    • FABP7 and Brn2 are proteins of interest in RCC research.

    Purpose of the Study:

    • To evaluate the efficacy of TMAs for analyzing RCC specimens.
    • To assess the agreement between TMA staining and conventional tissue slice staining for FABP7 and Brn2.
    • To determine the reliability of TMA analysis across different tumor sizes.

    Main Methods:

    • Immunohistochemical staining of 114 RCC paraffin-embedded specimens using anti-FABP7 and anti-Brn2 antibodies.
    • Comparison of staining results between TMA grafts and conventional tissue slice grafts.
    • Analysis of tumor staining area and agreement rates.

    Main Results:

    • FABP7 positive ratio was 74%; Brn2 positive ratio was 57%.
    • 100% agreement rate was observed for both antibodies between TMA and conventional slices.
    • Staining agreement was consistent irrespective of pre-extraction tumor size.

    Conclusions:

    • Immunostaining of TMA slices is a reliable and effective method for RCC specimen analysis.
    • TMAs offer a consistent approach for evaluating biomarkers like FABP7 and Brn2 in RCC.
    • This technique supports the potential for streamlined and accurate RCC diagnostics.