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Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells
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VERO stable cell lines expressing full-length human epidermal growth factor receptors 2 and 3: platforms for

Akbar Hedayatizadeh-Omran1, Reza Valadan1,2, Alireza Rafiei1,2

  • 11 Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences , Sari, Iran .

DNA and Cell Biology
|June 30, 2015
PubMed

Insights

Researchers developed specific cell lines to study resistance in breast cancer. These VERO cell lines expressing human epidermal growth factor receptors 2 and 3 (HER2 and HER3) enable new antibody development for targeted therapies.

Area of Science:

  • Oncology
  • Molecular Biology
  • Immunology

Background:

  • Cross-talk between human epidermal growth factor receptor 2 (HER2) and human epidermal growth factor receptor 3 (HER3) is implicated in therapeutic resistance in breast cancer.
  • Developing targeted therapies requires specific antibodies against HER2 and HER3.

Purpose of the Study:

  • To establish a cell line system for selecting conformation- and activation-specific antibodies against HER2 and HER3.
  • To provide a platform for subtractive phage display methods targeting HER2 and HER3.

Main Methods:

  • Utilized VERO cells for heterogeneous expression of HER2 and HER3.
  • Established stable VERO cell lines expressing high levels of HER2 and HER3, individually and in combination.
  • Assessed biological activity and heterodimerization of expressed HER2 and HER3 upon neuregulin1 treatment.

Main Results:

  • VERO cells are suitable for heterogeneous expression of HER2 and HER3.
  • Established VERO cell lines with controlled expression of HER2 and HER3.
  • Demonstrated that HER2 and HER3 expressed in VERO cells are biologically active and form heterodimers.

Conclusions:

  • The developed VERO cell lines provide a robust platform for phage display and antibody selection against HER2 and HER3.
  • These cell lines can be utilized for various HER-related research, aiding in understanding and overcoming therapeutic resistance in breast cancer.

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