Related Experiment Video
Updated: Apr 8, 2026

IridiumIII Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II
Published on: July 7, 2015
Hemicyanine dyes derived from 2,3,3-trimethyl-3H-indolium as candidates for non-covalent protein probes
Janina Kabatc1, Katarzyna Jurek1, Katarzyna Kostrzewska1
1UTP, University of Science and Technology, Faculty of Chemical Technology and Engineering, Seminaryjna 3, 85-326 Bydgoszcz, Poland.
Abstract:
Non-covalent interaction of hemicyanine dyes, derivatives of 2,3,3-trimethyl-3H-indolium with bovine serum albumin has been studied by spectral method. For this purpose, three hemicyanine dyes containing N-(2-carboxyethyl)-2,3,3-trimethyl-3H-indolium moiety were synthesized and their UV/Vis and fluorescence spectra, aggregation, photostability and association with bovine serum albumin were studied. The hemicyanine dyes with 2-ethylcarboxylic group was found to interact with bovine serum albumin, which is probably due to negative charge on the dye molecule at the expense of the carboxylic group and the ability to form hydrogen bonds with albumin.

