Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.8K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.8K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

12.2K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
12.2K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

22.1K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
22.1K
Atomic Force Microscopy01:08

Atomic Force Microscopy

4.7K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
4.7K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

15.2K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
15.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Extracting Axial Depth and Trajectory Trend Using Astigmatism, Gaussian Fitting, and CNNs for Protein Tracking.

Proceedings. IEEE International Symposium on Biomedical Imaging·2026
Same author

On the localization of the high-intensity region of simultaneous space-time foci.

Optics express·2025
Same author

Utilizing non-clonal CHO cell derived materials for preclinical studies of complex molecules.

BMC biotechnology·2025
Same author

A 3D-printed optical microscope for low-cost histological imaging.

Journal of microscopy·2025
Same author

Scalable spatiotemporal prediction with Bayesian neural fields.

Nature communications·2024
Same author

Bichromatic tetraphasic full-field optical coherence microscopy.

Journal of biomedical optics·2024
Same journal

Vogel spiral-based tilt-scan averaging approach for robust and efficient diffraction contrast suppression in DPC STEM.

Microscopy (Oxford, England)·2026
Same journal

Development of a specialized diamond knife for controlled notch introduction in ultrathin polymer films for in situ tensile transmission electron microscopy.

Microscopy (Oxford, England)·2026
Same journal

Study of nanocrystals within lamellar structures of polyvinylidene fluoride using phase plate scanning transmission electron microscopy.

Microscopy (Oxford, England)·2026
Same journal

Capability of angle-resolved SXES experiment examined by hexagonal BN and its application for the chemical bonding state of Fe2B.

Microscopy (Oxford, England)·2026
Same journal

Cryo-EELS elemental mapping of organic-solvent systems.

Microscopy (Oxford, England)·2026
Same journal

In-situ biasing DPC STEM observation of GaAs p-n junction.

Microscopy (Oxford, England)·2026
See all related articles

Related Experiment Video

Updated: Apr 8, 2026

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging
10:01

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging

Published on: September 8, 2017

8.3K

Aberrations and adaptive optics in super-resolution microscopy.

Martin Booth1, Débora Andrade2, Daniel Burke2

  • 1Centre for Neural Circuits and Behaviour, University of Oxford, Tinsley Building, Mansfield Road, Oxford OX1 3SR, UK Department of Engineering Science, University of Oxford, Parks Road, Oxford OX1 3PJ, UK martin.booth@eng.ox.ac.uk.

Microscopy (Oxford, England)
|July 1, 2015
PubMed
Summary
This summary is machine-generated.

Super-resolution microscopy offers nanoscale biological insights but suffers from optical aberrations. Adaptive optics effectively corrects these aberrations, enhancing imaging quality in techniques like single-molecule switching and stimulated emission depletion.

Keywords:
aberrationsadaptive opticssingle-molecule switchingstimulated emission depletionstructured illuminationsuper-resolution microscopy

More Related Videos

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

10.7K
Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline
09:14

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline

Published on: September 13, 2022

3.2K

Related Experiment Videos

Last Updated: Apr 8, 2026

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging
10:01

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging

Published on: September 8, 2017

8.3K
Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

10.7K
Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline
09:14

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline

Published on: September 13, 2022

3.2K

Area of Science:

  • Biophysics
  • Cell Biology
  • Optical Imaging

Background:

  • Fluorescence microscopy is crucial for studying cellular structures and dynamics.
  • Super-resolution microscopy (nanoscopy) provides nanoscale resolution in living cells.
  • Optical aberrations significantly degrade the performance of advanced microscopy techniques.

Purpose of the Study:

  • To review the impact of optical aberrations on super-resolution microscopy.
  • To discuss how different super-resolution techniques are affected by aberrations.
  • To highlight adaptive optics as a solution for aberration correction.

Main Methods:

  • Review of super-resolution microscopy techniques: single-molecule switching, stimulated emission depletion, and structured illumination.
  • Analysis of aberration effects specific to each technique.
  • Discussion of adaptive optics principles and applications in microscopy.

Main Results:

  • Super-resolution methods exhibit distinct sensitivities to optical aberrations.
  • Aberrations limit the achievable resolution and image quality.
  • Adaptive optics can effectively compensate for optical aberrations.

Conclusions:

  • Optical aberrations are a major challenge for super-resolution microscopy.
  • Adaptive optics presents a viable strategy to overcome these limitations.
  • Improving aberration correction will further advance nanoscale imaging in biology.