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Characterization of a methyl-specific restriction system in Clostridium acetobutylicum strain N1-4081
H Azeddoug1, J Hubert, G Reysset
1Unité des Anaérobies, Institut Pasteur, Paris, France.
FEMS Microbiology Letters
|December 1, 1989
Abstract:
A type II restriction endonuclease, named CacI, was detected in Clostridium acetobutylicum strain N1-4081. CacI cleaved the tetranucleotide sequence [5' decreases -GATC-3']. The modification system consisted of the methylation of the adenine present in this sequence. CacI, an isoschizomer of MboI, is inactive on dam methylated substrates.