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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Apr 7, 2026

Flow Cytometric Analysis of Multiple Mitochondrial Parameters in Human Induced Pluripotent Stem Cells and Their Neural and Glial Derivatives
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High Throughput-Based Mitochondrial Function Assays by Multi-Parametric Flow Cytometry.

J Paul Robinson1, Nianyu Li2, Padma Kumar Narayanan2

  • 1Purdue University Cytometry Laboratories, West Lafayette, Indiana.

Current Protocols in Cytometry
|July 2, 2015
PubMed
Summary
This summary is machine-generated.

This study presents a flow cytometry assay to evaluate chemical-induced mitochondrial dysfunction. The assay simultaneously measures key cellular parameters to assess toxicity mechanisms.

Keywords:
cell viabilityflow cytometrymitochondriamitochondrial dysfunctionmitochondrial membrane potentialreactive oxygen species (ROS)

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Area of Science:

  • Toxicology
  • Cell Biology
  • Biochemistry

Background:

  • Mitochondrial dysfunction is a key mechanism in chemical toxicity.
  • Assessing mitochondrial function is crucial for understanding cellular responses to xenobiotics.

Purpose of the Study:

  • To describe a multi-parametric flow cytometry assay for evaluating drug or chemical-induced mitochondrial dysfunction.
  • To provide a method for simultaneous measurement of critical cellular and mitochondrial parameters.

Main Methods:

  • Cells cultured in glucose-supplemented medium and exposed to increasing chemical concentrations.
  • Multi-parametric flow cytometry assay to measure mitochondrial membrane potential (MMP), reactive oxygen species (ROS) production, reduced glutathione (GSH) levels, and cell viability.
  • Simultaneous assessment of multiple parameters indicative of mitochondrial dysfunction.

Main Results:

  • The assay allows for the simultaneous measurement of key mitochondrial and cellular parameters.
  • This method can detect and quantify chemical-induced alterations in mitochondrial function and cell viability.
  • The assay provides a comprehensive profile of mitochondrial toxicity.

Conclusions:

  • The described flow cytometry assay is a valuable tool for assessing chemical-induced mitochondrial dysfunction.
  • This multi-parametric approach offers a robust method for toxicological evaluations.
  • The assay facilitates a deeper understanding of the mechanisms underlying chemical toxicity.