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Related Experiment Video

Updated: Apr 7, 2026

Author Spotlight: Exploring Peripheral Mechanisms of Neuropathic Pain in Trigeminal Nerve Injury

Author Spotlight: Exploring Peripheral Mechanisms of Neuropathic Pain in Trigeminal Nerve Injury

Published on: February 9, 2024

Synaptic ultrastructure changes in trigeminocervical complex posttrigeminal nerve injury.

John Park¹, Van Nancy Trinh², Ilse Sears-Kraxberger³

¹Department of Pharmacology, University of California Irvine, School of Medicine, Irvine, California, 92697.

The Journal of Comparative Neurology

|July 2, 2015

Summary

Injury to the infraorbital nerve in rats caused more excitatory synapses in the brainstem's trigeminocervical complex, contributing to orofacial neuropathic pain.

Keywords:

RRID:RGD_70508 RRID:nif-0000-30467 RRID:rid_000081 orofacial neuropathic pain synaptic ultrastructure trigeminal nerve injury

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Last Updated: Apr 7, 2026

Author Spotlight: Exploring Peripheral Mechanisms of Neuropathic Pain in Trigeminal Nerve Injury

Author Spotlight: Exploring Peripheral Mechanisms of Neuropathic Pain in Trigeminal Nerve Injury

Published on: February 9, 2024

Author Spotlight: Utilizing Infraorbital Nerve Ligation in Mice for Investigating Trigeminal Neuropathic Pain and Treatment Strategies

Author Spotlight: Utilizing Infraorbital Nerve Ligation in Mice for Investigating Trigeminal Neuropathic Pain and Treatment Strategies

Published on: March 8, 2024

Author Spotlight: Regenerative Roles of Muscle Proteins at Neuromuscular Junction Post-Nerve Injury

Author Spotlight: Regenerative Roles of Muscle Proteins at Neuromuscular Junction Post-Nerve Injury

Published on: November 1, 2024

Area of Science:

Neuroscience
Pain Research
Cellular Biology

Background:

The trigeminocervical complex (Vc/C2) processes orofacial sensory information.
Trigeminal nerve injury can lead to chronic pain through synaptic changes.

Purpose of the Study:

To investigate if infraorbital nerve injury causes synaptic ultrastructural changes in the Vc/C2.
To correlate these changes with neuropathic pain states.

Main Methods:

Chronic constriction injury to the infraorbital nerve (CCI-ION) in rats.
Transmission electron microscopy to analyze synaptic profiles in Vc/C2.
Classification and quantification of excitatory (R-), inhibitory (F-), and primary afferent (C-) terminals.

Main Results:

Increased numbers of excitatory (R-) synaptic profiles were observed in the superficial dorsal horn of Vc/C2.
Decreased mean synaptic contact length of R-profiles was noted.
These changes occurred 3 weeks post-CCI-ION, coinciding with peak hypersensitivity.

Conclusions:

Orofacial neuropathic pain is associated with an increase in excitatory synapses in the Vc/C2.
Enhanced excitatory neurotransmission in the Vc/C2 may contribute to the development of orofacial pain states.