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Published on: April 9, 2012
Comparison of Three Commercial Molecular Assays for Detection of Rifampin and Isoniazid Resistance among
K Strydom1, F Ismail2, M M Z Matabane2
1Department of Medical Microbiology, University of Pretoria, Pretoria, South Africa National Health Laboratory Services, Tshwane Academic Division, Pretoria, South Africa strydom.kathy@gmail.com.
Abstract:
In a head-to-head comparison of the MTBDRplus version 2.0 (Hain Lifescience), the Xpert MTB/RIF (Cepheid), and the Anyplex MTB/NTM (Seegene) assays, we demonstrated equal sensitivity (59/61; 96.7%) and specificity (53/54; 98.1%) for detecting rifampin resistance with further analysis of discordances. The Xpert assay does not detect isoniazid resistance while the Anyplex assay showed high false positivity.

