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Proteomic Analysis Reveals Aberrant O-GlcNAcylation of Extracellular Proteins from Breast Cancer Cell Secretion

Pukkavadee Netsirisawan1, Daranee Chokchaichamnankit2, Chantragan Srisomsap3

  • 1Applied Biological Sciences Program, Chulabhorn Graduate Institute, Laksi, Bangkok, Thailand.

Cancer Genomics & Proteomics
|July 3, 2015
PubMed
Abstract

Insights

Aberrant O-GlcNAcylation was found in secreted proteins from breast cancer cells. This study identified specific O-GlcNAc-modified proteins, like HSP70 and TER ATPase, in cancer cell secretions, suggesting potential breast cancer biomarkers.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cancer Research

Background:

  • O-GlcNAcylation is a key intracellular protein modification.
  • Aberrant O-GlcNAcylation is observed in breast cancer tissues.
  • Extracellular O-GlcNAc-modified proteins are poorly understood.

Purpose of the Study:

  • To investigate aberrant secretion of O-GlcNAc-modified proteins from breast cancer cells.
  • To identify specific extracellular O-GlcNAc-modified proteins in breast cancer.
  • To explore potential biomarkers for breast cancer detection.

Main Methods:

  • Preparation of intracellular and extracellular proteins from breast cancer and normal cells.
  • Examination of O-GlcNAcylation levels using immunoblotting.
  • Identification of O-GlcNAc-modified proteins via 2D gel electrophoresis and LC-MS/MS.

Main Results:

  • Significantly increased extracellular O-GlcNAcylation in breast cancer cells compared to normal cells.
  • Distinct O-GlcNAc patterns between intracellular and extracellular proteins.
  • Identification of increased O-GlcNAc-modified transitional endoplasmic reticulum ATPase (TER ATPase) and heat-shock 70 kDa (HSP70) in cancer cell secretions.

Conclusions:

  • Extracellular O-GlcNAcomic studies reveal aberrant secretion of O-GlcNAc-modified proteins in breast cancer.
  • O-GlcNAc-modified HSP70 and TER ATPase are elevated in breast cancer cell secretions.
  • These findings suggest potential utility of extracellular O-GlcNAc-modified proteins as breast cancer biomarkers.
Keywords:
Breast cancerO-GlcNAcylationextracellular proteinsheat-shock 70 kDatransitional endoplasmic reticulum ATPase

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