Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

22.1K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
22.1K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.8K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.8K
Atomic Force Microscopy01:08

Atomic Force Microscopy

4.7K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
4.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Duration of Care in Early Intervention in Psychosis Services: A Multi-Perspective Qualitative Study.

Early intervention in psychiatry·2026
Same author

Metabolic dysfunction in multiple sclerosis: Elevated lactate and impaired post-exercise creatine response in the anterior cingulate cortex.

Multiple sclerosis and related disorders·2026
Same author

Using functional MRI neurofeedback to modulate self-blame in major depressive disorder: A pilot study.

NeuroImage. Clinical·2026
Same author

From early intervention in psychosis to intensive care: correlates of restrictive psychiatric practice in a national retrospective cohort study.

The British journal of psychiatry : the journal of mental science·2026
Same author

A randomised feasibility trial of an intervention involving mental health support workers as link workers to improve dental visiting in people with severe mental illness: The Mouth Matters in Mental Health Study.

Health and social care delivery research·2026
Same author

High content 3D imaging by dual-view oblique plane microscopy.

PNAS nexus·2025

Related Experiment Video

Updated: Apr 7, 2026

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
09:19

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

Published on: August 29, 2025

716

Fibre-coupled multiphoton microscope with adaptive motion compensation.

Ben Sherlock1, Sean Warren1, James Stone2

  • 1Department of Physics, Imperial College London, London, SW7 2AZ, UK.

Biomedical Optics Express
|July 3, 2015
PubMed
Summary

This study introduces a new multiphoton microscope that actively compensates for sample movement during in vivo imaging. The system successfully corrects motion up to 700 µm/s, improving image quality.

Keywords:
(180.4315) Nonlinear microscopy(330.4150) Motion detection

More Related Videos

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.5K
Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

10.4K

Related Experiment Videos

Last Updated: Apr 7, 2026

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
09:19

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

Published on: August 29, 2025

716
Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.5K
Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

10.4K

Area of Science:

  • Biomedical Optics
  • Microscopy Engineering
  • In Vivo Imaging

Background:

  • In vivo imaging is crucial for biological research but is often limited by sample motion.
  • Existing microscopy techniques struggle to maintain focus and image quality when samples move axially.

Purpose of the Study:

  • To develop and validate a fibre-coupled multiphoton microscope with active axial motion compensation.
  • To overcome the challenges posed by sample movement during in vivo microscopy.

Main Methods:

  • A fibre-coupled multiphoton microscope was integrated with optical coherence tomography (OCT) for surface tracking.
  • A piezo actuator was used for real-time axial adjustment of the objective lens based on OCT feedback.
  • System performance was characterized, and its effectiveness was demonstrated in ex vivo mouse skin imaging.

Main Results:

  • The developed system demonstrates effective axial motion compensation.
  • The microscope can compensate for sample axial velocities up to 700 µm/s.
  • Motion compensation significantly improved image quality of multiphoton excited autofluorescence in mouse skin.

Conclusions:

  • Active axial motion compensation is a viable strategy for improving in vivo multiphoton microscopy.
  • The developed fibre-coupled system offers enhanced stability and image fidelity for dynamic biological samples.
  • This technology has potential applications in various fields requiring high-resolution in vivo imaging.