Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

17.5K
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
17.5K
Antigen Presenting Cells01:22

Antigen Presenting Cells

4.4K
The immune system is a complex network of cells and molecules that protects the body from foreign invaders. T cells, a type of white blood cell, play a crucial role in this process. They recognize and attack foreign substances, such as pathogens, that enter the body.
T cells require the help of antigen-presenting cells (APCs), which process foreign antigens into smaller fragments that can be recognized by T cells. These APCs are highly specialized cells that efficiently internalize antigens...
4.4K
Antigens Involved in Adaptive Immunity01:26

Antigens Involved in Adaptive Immunity

1.9K
An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and...
1.9K
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

18.5K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
18.5K
Protein Glycosylation01:25

Protein Glycosylation

10.5K
Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...
10.5K
Cell-mediated Immune Responses01:40

Cell-mediated Immune Responses

86.9K
Overview
86.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impaired emotional response to stress in mice lacking galectin-1 or galectin-3.

Physiology & behavior·2020
Same author

Dynamic intracellular delivery of antibiotics via pH-responsive polymersomes.

Polymer chemistry·2015
Same author

Trichuris suis soluble products induce Rab7b expression and limit TLR4 responses in human dendritic cells.

Genes and immunity·2015
Same author

Selectin ligands promote ultrasound contrast agent adhesion under shear flow.

Molecular pharmaceutics·2006
Same author

Dimerization of a selectin and its ligand stabilizes cell rolling and enhances tether strength in shear flow.

Proceedings of the National Academy of Sciences of the United States of America·2001
Same author

Antibodies to glycans dominate the host response to schistosome larvae and eggs: is their role protective or subversive?

The Journal of infectious diseases·2001
Same journal

Stable, micron-scale lipocondensates achieving prolonged circulation without PEGlyated lipids.

Biomaterials science·2026
Same journal

Fiber and continuum scale contributions to the intrinsic and apparent fracture of soft collagenous tissue <i>via</i> cutting.

Biomaterials science·2026
Same journal

Surface morphology-regulated tissue adhesion in solid and mesoporous silica-reinforced gelatin nanocomposite hydrogels.

Biomaterials science·2026
Same journal

Nanostructured hyaluronic acid-chia mucilage film as bioactive wound dressings for accelerated skin regeneration.

Biomaterials science·2026
Same journal

Tunable bio-inspired hybrid hydrogels reprogram stem cell-derived extracellular vesicles for superior wound regeneration.

Biomaterials science·2026
Same journal

Bioorthogonally reinforced injectable granular hydrogels synergizing ECM mimicry with microporosity for skin tissue engineering.

Biomaterials science·2026
See all related articles

Related Experiment Video

Updated: Apr 7, 2026

Author Spotlight: Dendritic Cells Maturation Using Sialidases-Based Enzymatic Treatment of the Cell Surface
13:36

Author Spotlight: Dendritic Cells Maturation Using Sialidases-Based Enzymatic Treatment of the Cell Surface

Published on: October 20, 2023

4.4K

Presentation Modality of Glycoconjugates Modulates Dendritic Cell Phenotype.

N A Hotaling1, D M Ratner2, R D Cummings3

  • 1Wallace H. Coulter Dept. of Biomedical Engineering, Georgia Institute of Technology, Atlanta GA, 30332.

Biomaterials Science
|July 7, 2015
PubMed
Summary
This summary is machine-generated.

Dendritic cell (DC) responses to glycoconjugates vary significantly based on how they are presented. Adsorbed glycoconjugates are most inflammatory, while soluble ones are least, impacting therapeutic development.

More Related Videos

An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets
09:09

An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets

Published on: April 18, 2016

16.0K
Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes
06:09

Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes

Published on: June 22, 2016

24.2K

Related Experiment Videos

Last Updated: Apr 7, 2026

Author Spotlight: Dendritic Cells Maturation Using Sialidases-Based Enzymatic Treatment of the Cell Surface
13:36

Author Spotlight: Dendritic Cells Maturation Using Sialidases-Based Enzymatic Treatment of the Cell Surface

Published on: October 20, 2023

4.4K
An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets
09:09

An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets

Published on: April 18, 2016

16.0K
Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes
06:09

Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes

Published on: June 22, 2016

24.2K

Area of Science:

  • Immunology
  • Glycobiology
  • Biotechnology

Background:

  • Dendritic cell (DC) response to glycoconjugates is critical for vaccine and therapeutic development.
  • Current methods using microarrays may not accurately predict in vivo responses due to differing presentation modalities.
  • Understanding how DCs interact with glycans in soluble, phagocytosable, or immobilized forms is crucial.

Purpose of the Study:

  • To investigate and compare dendritic cell (DC) functional responses to glycoconjugates presented in various modalities.
  • To assess the impact of glycoconjugate display (soluble, phagocytosable, adsorbed) on DC phenotypes.
  • To bridge the gap between microarray data and functional cellular responses.

Main Methods:

  • Mannose or glucose conjugated to cationized bovine serum albumin were used as model glycoconjugates.
  • Glycoconjugates were presented to DCs in soluble, phagocytosable, and adsorbed (non-phagocytosable) forms.
  • High-throughput assays and multivariate general linear models (GLM) analyzed DC phenotypic outcomes.

Main Results:

  • Statistically significant differences in DC responses were observed across different display modalities.
  • Adsorbed glycoconjugates induced the most pro-inflammatory DC responses, while soluble conjugates induced the least.
  • Mannose-glycoconjugate interactions were calcium-dependent and inhibited by anti-DC-SIGN antibodies.

Conclusions:

  • The presentation modality of glycoconjugates profoundly influences dendritic cell (DC) activation and phenotype.
  • Findings suggest that microarray data may not directly translate to therapeutic efficacy without considering presentation context.
  • This study provides a framework for understanding glycan-based immunomodulation and designing more effective glycan-based therapeutics.