Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Surface-Enhanced Raman Spectroscopy of Fluid-Supported Lipid Bilayers.

ACS applied materials & interfaces·2019
Same author

Advances in surface-enhanced Raman spectroscopy (SERS) substrates for lipid and protein characterization: sensing and beyond.

The Analyst·2018
Same author

The facile removal of CTAB from the surface of gold nanorods.

Colloids and surfaces. B, Biointerfaces·2018
Same author

Ultrasensitive and towards single molecule SERS: general discussion.

Faraday discussions·2017
Same author

Theory of SERS enhancement: general discussion.

Faraday discussions·2017
Same author

Novel Liposome-Based Surface-Enhanced Raman Spectroscopy (SERS) Substrate.

The journal of physical chemistry letters·2017

Related Experiment Video

Updated: Apr 7, 2026

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
09:09

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

9.2K

Localized Surface Plasmon Resonance Biosensing: Current Challenges and Approaches.

Sarah Unser1, Ian Bruzas2, Jie He3

  • 1Department of Chemistry, College of Arts and Sciences, University of Cincinnati, 301 West Clifton Court, Cincinnati, OH 45221-0172, USA. unsersa@mail.uc.edu.

Sensors (Basel, Switzerland)
|July 7, 2015
PubMed
Summary

Localized surface plasmon resonance (LSPR) biosensing offers sensitive detection but faces challenges in sensitivity, selectivity, membrane-associated species detection, and point-of-care adaptation. This review highlights recent advances to overcome these limitations for improved diagnostic applications.

Keywords:
biosensingnoble metal nanoparticlesplasmonicpoint-of-care diagnostics

More Related Videos

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

21.6K
Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas
10:43

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

Published on: July 21, 2023

4.3K

Related Experiment Videos

Last Updated: Apr 7, 2026

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
09:09

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

9.2K
Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

21.6K
Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas
10:43

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

Published on: July 21, 2023

4.3K

Area of Science:

  • Nanotechnology
  • Biomedical Engineering
  • Analytical Chemistry

Background:

  • Localized Surface Plasmon Resonance (LSPR) is a label-free biosensing technique known for its sensitivity, robustness, and ease of use.
  • LSPR biosensing relies on detecting shifts in plasmon frequency due to changes in the local refractive index near nanoparticle surfaces.
  • While widely adopted for biomolecular interaction analysis, LSPR biosensing faces persistent challenges.

Purpose of the Study:

  • To categorize and review the key challenges in LSPR biosensing.
  • To discuss recent advancements and strategies for overcoming these limitations.
  • To explore the future of LSPR for point-of-care diagnostics.

Main Methods:

  • Conceptual discussion of LSPR principles and optical signal detection factors.
  • Review of recent literature on LSPR biosensing applications and improvements.
  • Categorization of challenges into sensitivity/limit of detection, selectivity, membrane-associated species detection, and point-of-care adaptation.

Main Results:

  • Improvements in limit of detection achieved through various amplification strategies.
  • Enhanced selectivity in complex biological solutions using self-assembled monolayers, plasmon rulers, and shape complementarity.
  • Development of LSPR platforms for sensitive detection of membrane-associated species.
  • Advances in multiplexed and microfluidic LSPR devices for rapid, point-of-care diagnostics.

Conclusions:

  • Recent innovations address critical limitations in LSPR biosensing, enhancing sensitivity and selectivity.
  • New platforms are emerging for detecting challenging analytes like membrane-associated species.
  • The field is progressing towards practical, cost-effective, and rapid point-of-care diagnostic devices.