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Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
Published on: March 20, 2015
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Advances in surface-enhanced Raman spectroscopy (SERS) substrates for lipid and protein characterization: sensing and
Ian Bruzas1, William Lum, Zohre Gorunmez
1Department of Chemistry, University of Cincinnati, 301 Clifton Court, Cincinnati, OH 45221, USA. saglela@ucmail.uc.edu.
The Analyst
|July 31, 2018
Summary
Surface-enhanced Raman spectroscopy (SERS) provides label-free detection of biomolecules. Advances in SERS substrates enable detailed biophysical characterization of proteins, lipids, and cells.
Area of Science:
- Analytical Chemistry
- Biophysics
- Spectroscopy
Background:
- Surface-enhanced Raman spectroscopy (SERS) is a powerful ultrasensitive technique for biomolecular analysis.
- It allows label-free, direct detection of molecules via their unique Raman fingerprints.
- Characterizing dynamic structures like protein and lipid bilayers requires non-perturbative methods.
Purpose of the Study:
- To review the applications of SERS in biomolecular analysis.
- To discuss advancements in SERS substrate development.
- To highlight biophysical insights gained from SERS studies of biomolecules and cells.
Main Methods:
- Discussion of various SERS substrate categories (solution-phase, solid-supported, tip-enhanced Raman spectroscopy (TERS), single-molecule).
- Review of SERS applications for detecting proteins and biological lipid membranes.
- Analysis of biophysical characterization of proteins, lipids, and live cells using SERS.
Main Results:
- SERS enables label-free quantitation and structural analysis of biomolecules.
- Developed SERS substrates overcome challenges in non-perturbative measurements.
- SERS provides significant biophysical insights into complex biological systems.
Conclusions:
- SERS is an essential tool for ultrasensitive biomolecular analysis.
- Advances in SERS substrates have broadened its applicability.
- SERS offers immense potential for understanding biomolecular interactions and cellular activity.
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