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MYCNOS functions as an antisense RNA regulating MYCN
Nadia Vadie1, Sheena Saayman, Alexandra Lenox
1a Molecular and Experimental Medicine ; The Scripps Research Institute ; La Jolla , CA USA.
RNA Biology
|July 10, 2015
Summary
Neuroblastoma
Area of Science:
- Molecular biology
- Cancer research
- Epigenetics
Background:
- Neuronal MYC (MYCN) amplification correlates with poor neuroblastoma prognosis.
- Antisense transcripts, like MYCNOS, originate from the opposite strand of MYCN.
- Long non-coding RNAs (lncRNAs) are implicated in epigenetic gene regulation.
Purpose of the Study:
- To investigate the role of MYCNOS transcripts in regulating the MYCN locus.
- To determine if MYCNOS affects MYCN promoter usage and expression.
- To explore the potential of protein-coding transcripts in gene transcription regulation.
Main Methods:
- Analysis of MYCNOS transcript function.
- Investigation of MYCN promoter activity.
- Protein recruitment studies involving G3BP1.
Main Results:
- MYCNOS transcripts modulate the MYCN locus, impacting MYCN promoter usage.
- MYCNOS recruits proteins, such as G3BP1, to the upstream MYCN promoter.
- Overexpression of MYCNOS reduces upstream MYCN promoter usage and increases MYCN expression.
Conclusions:
- Protein-coding MYCNOS acts as a regulator of MYCN transcription.
- MYCNOS controls MYCN transcriptional variants and affects neuroblastoma prognosis.
- Protein-coding transcripts can regulate gene transcription and tether regulatory proteins.
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