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Modified toolbox for optogenetics in the nonhuman primate.

Ji Dai1, Ilker Ozden2, Daniel I Brooks1

  • 1Brown University , Department of Neuroscience, 185 Meeting Street, Box GL-N, Providence, Rhode Island 02912, United States.

Neurophotonics
|July 10, 2015
PubMed
Summary

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This summary is machine-generated.

This study presents a reliable optogenetics methodology for nonhuman primate research, improving viral transduction and optical stimulation for consistent neural modulation. These advancements enable precise control of neural circuits for research and potential clinical applications.

Area of Science:

  • Neuroscience
  • Optogenetics
  • Primate Research

Background:

  • Optogenetics offers significant advantages for neuroscience research.
  • Implementing optogenetics in nonhuman primate models presents challenges in cell transduction and monitoring neural activity.
  • Existing methods often lack reliability and can cause significant tissue damage.

Purpose of the Study:

  • To describe a refined optogenetics methodology for reliable application in nonhuman primates.
  • To detail an "in-chair" viral injection system and optimized optical fibers.
  • To demonstrate the efficacy and replicability of the developed methods.

Main Methods:

  • Development of an "in-chair" viral injection system for precise delivery in monkey brains.
  • Utilization of tapered and thinner optical fibers for reduced tissue damage and enhanced stimulation efficacy.
Keywords:
methodologynonhuman primateoptogenetics

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  • Application of the methodology in both deep and shallow cortical areas of multiple monkeys.

    • Main Results:

    • Successful and consistent transduction of cells in various cortical regions of nonhuman primates.
    • Sustained neural modulation for several months post-injection without light-induced artifacts during recordings.
    • Demonstration of optogenetic stimulation's ability to bias spatial attention, comparable to electrical microstimulation.

    Conclusions:

    • The developed optogenetics methodology is reliable, replicable, and minimizes tissue damage in nonhuman primates.
    • This technique allows for long-term neural modulation and precise control of neural circuits.
    • The methodology holds promise for advancing fundamental neuroscience research and clinical applications.