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Evaluation environment for digital and analog pathology: a platform for validation studies.

Brandon D Gallas1, Marios A Gavrielides1, Catherine M Conway2

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This study introduces eeDAP, a platform comparing digital whole slide images (WSIs) to traditional glass slides for pathology interpretation. eeDAP enables direct comparison of the same regions, improving accuracy in digital pathology studies.

Keywords:
digital pathologymicroscopyreader studiestechnology evaluationvalidationwhole slide imaging

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Area of Science:

  • Digital Pathology
  • Histopathology Analysis
  • Medical Imaging

Background:

  • Pathologists interpret histopathology slides using optical microscopes or digital whole slide images (WSIs).
  • Comparing interpretations between digital and analog pathology is crucial for validating new technologies.
  • Discrepancies can arise from interpreting different regions or variations in image quality.

Purpose of the Study:

  • To present eeDAP (evaluation environment for digital and analog pathology), a platform for comparing pathologist interpretations of WSIs versus glass slides.
  • To enable direct comparison of identical regions of interest (ROIs) between digital and analog pathology domains.
  • To facilitate the evaluation of image quality and individual cell morphology in both digital and analog formats.

Main Methods:

  • Developed eeDAP, an evaluation environment integrating computer-controlled microscopy with WSI registration.
  • Utilized a camera on the microscope to capture real-time field of view (FOV) images, enabling precise WSI registration to glass slides.
  • Reduced interpretation areas from whole slides to small ROIs for focused comparison.

Main Results:

  • Achieved registration of WSIs to glass slides, allowing evaluation of the same ROIs across both formats.
  • Enabled comparison of image quality and interpretation of individual cells between digital and analog pathology.
  • Provided calculations and images demonstrating the registration accuracy and ROI extraction process.

Conclusions:

  • eeDAP facilitates rigorous comparison between digital and analog pathology interpretation by ensuring identical regions are evaluated.
  • The platform aids in assessing image quality and reducing interpretation variability in digital pathology.
  • eeDAP software is available as a free, downloadable MATLAB source or standalone application.