Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Tight Junctions

Tight Junctions

Tight junctions are molecular seals between cells that prevent the leaking of fluids, ions, and other small solutes across cavities and compartments in multicellular organisms. They are mainly composed of claudin and occludin transmembrane proteins, and other proteins such as tricellulin and JAM (junctional adhesion molecule). All these proteins are 4-pass transmembrane proteins, except JAM, which is a single-pass transmembrane protein belonging to the immunoglobulin superfamily. The...

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...

Overview of Cell-Cell Junctions

Overview of Cell-Cell Junctions

The complex three-dimensional arrangement of cells in any multicellular organism is defined and maintained by interactions of cells with each other and the extracellular matrix. Cell-cell junctions are specialized structures where the multi-protein complexes on one cell interact with the multi-protein complexes on another cell. These cell junctions are classified into three main types based on their function — occluding, anchoring, and gap junctions.
Occluding or Tight...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Mechanics-driven emergence of mesenchymal migration features.

Biomechanics and modeling in mechanobiology·2026

Same author

Mapping the evidence on exercise-related ventricular-arterial coupling adaptations in male adults: a scoping review.

Frontiers in cardiovascular medicine·2026

Same author

N-acetylcysteine exerts senescence-selective antifibrotic effects in fibroblast from chronic wounds in Recessive Dystrophic Epidermolysis Bullosa.

The British journal of dermatology·2026

Same author

Fibronectin matrix remodelling modulates the active nematic dynamics of cancer-associated fibroblasts.

Nature materials·2026

Same author

Are Wearable Sleep-Tracking Devices Reliable Alternatives to Polysomnography? A Systematic Review and Meta-Analysis.

Behavioral sleep medicine·2026

Same author

Modeling cell migratory persistence through temporal correlations and angular noise.

PloS one·2026

Same journal

Chlorinated VSLSs Surpass HCFCs in CFC-11-Equivalent Emissions for Ozone Layer Depletion in China.

Nature communications·2026

Same journal

Author Correction: Charge transfer in triphenylamine-tetrazine covalent organic frameworks for solar-driven hydrogen peroxide production.

Nature communications·2026

Same journal

Vegetation browning patterns under compound soil and atmospheric dryness in northern permafrost ecosystems.

Nature communications·2026

Same journal

Voltage imaging of CA1 pyramidal cells and SST+ interneurons reveals stability and plasticity mechanisms of spatial firing.

Nature communications·2026

Same journal

Radical-omics reveals the hydrogen-abstraction pathway of isoprene oxidation.

Nature communications·2026

Same journal

Toughening elastomer via sequentially activated multi-pathway energy dissipation.

Nature communications·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 7, 2026

An Intravital Microscopy-Based Approach to Assess Intestinal Permeability and Epithelial Cell Shedding Performance

An Intravital Microscopy-Based Approach to Assess Intestinal Permeability and Epithelial Cell Shedding Performance

Published on: December 3, 2020

Gap geometry dictates epithelial closure efficiency.

Andrea Ravasio¹, Ibrahim Cheddadi², Tianchi Chen¹

¹Mechanobiology Institute, National University of Singapore, Singapore 117411, Singapore.

Nature Communications

|July 10, 2015

Summary

This summary is machine-generated.

Epithelial tissue repair relies on cell crawling and actomyosin cable constriction, with gap geometry dictating their interplay. Mechanical coupling between these processes ensures efficient wound closure and tissue integrity restoration.

More Related Videos

An Epithelial Abrasion Model for Studying Corneal Wound Healing

An Epithelial Abrasion Model for Studying Corneal Wound Healing

Published on: December 29, 2021

Confocal Microscope-Based Laser Ablation and Regeneration Assay in Zebrafish Interneuromast Cells

Confocal Microscope-Based Laser Ablation and Regeneration Assay in Zebrafish Interneuromast Cells

Published on: May 20, 2020

Related Experiment Videos

Last Updated: Apr 7, 2026

An Intravital Microscopy-Based Approach to Assess Intestinal Permeability and Epithelial Cell Shedding Performance

An Intravital Microscopy-Based Approach to Assess Intestinal Permeability and Epithelial Cell Shedding Performance

Published on: December 3, 2020

An Epithelial Abrasion Model for Studying Corneal Wound Healing

An Epithelial Abrasion Model for Studying Corneal Wound Healing

Published on: December 29, 2021

Confocal Microscope-Based Laser Ablation and Regeneration Assay in Zebrafish Interneuromast Cells

Confocal Microscope-Based Laser Ablation and Regeneration Assay in Zebrafish Interneuromast Cells

Published on: May 20, 2020

Area of Science:

Cell biology
Biophysics
Developmental biology

Background:

Tissue integrity is crucial for multicellular organisms.
Misregulated tissue repair can cause inflammation and cancer.
Epithelial cells use crawling and actomyosin cable constriction to close gaps.

Purpose of the Study:

To investigate how epithelial gap geometry influences collective cell motion.
To elucidate the mechanical coupling between cell crawling and actomyosin cable contraction.
To model the roles of these mechanisms in tissue repair.

Main Methods:

In vitro and in vivo experiments on epithelial gap closure.
Analysis of collective cell migration dynamics.
Computational modeling of tissue repair mechanisms.

Main Results:

Epithelial gap geometry is a key regulator of cell crawling and actomyosin cable constriction.
Mechanical coupling between these two processes controls large-scale gap closure dynamics.
Computational models successfully clarified the distinct roles of each mechanism.

Conclusions:

A robust and universal mechanism for epithelial tissue repair has been identified.
Understanding this coupling provides insights into development and disease.
Gap geometry and mechanical feedback are critical for restoring tissue integrity.