Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transfer RNA Synthesis02:35

Transfer RNA Synthesis

4.0K
4.0K
Transfer RNA Synthesis02:36

Transfer RNA Synthesis

13.8K
One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
13.8K
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

15.3K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
15.3K
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

4.8K
4.8K
Directing Proteins to the Rough Endoplasmic Reticulum01:34

Directing Proteins to the Rough Endoplasmic Reticulum

18.4K
The organelle-specific signaling sequences direct proteins synthesized in the cytosol to their final destination like ER, mitochondria, peroxisomes, etc. Some of the proteins directed to ER are then trafficked via vesicles to other organelles within the cell or the extracellular environment through the Golgi complex. For example, the rough ER synthesizes soluble proteins for transportation to the lysosomes or secretion out of the cell. It can also synthesize transmembrane proteins that can...
18.4K
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

4.3K
4.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Extracellular flux analyses indicate low ATP yield and require refinement for accurate determination of maximal oxygen consumption rate.

Scientific reports·2026
Same author

A RiboCancer cell line panel reveals that CLL-associated Rps15 mutations translationally rewire transcription through codon-specific tRNA accommodation defects.

HemaSphere·2026
Same author

Dynamics of the β-cardiac myosin auto-inhibited state explain cardiomyopathy pathogenesis.

Nature communications·2026
Same author

Genomic newborn screening as a paradigm shift in rare disease management, with emphasis on endocrine conditions.

Annales d'endocrinologie·2026
Same author

Immune landscape characterization of neurofibromas with atypical features in Neurofibromatosis1 reveals PD-1 and the Tim-3/Galectin-9 pathway as potential therapeutic targets.

Acta neuropathologica communications·2026
Same author

Phenotype of neurofibromatosis 1: A comparison study between NF1 mutant mice and humans.

The Journal of investigative dermatology·2026
Same journal

Corrigendum: Inhibition of Myc family proteins eradicates KRas-driven lung cancer in mice.

Genes & development·2026
Same journal

A new perspective on ATR's role in translesion synthesis.

Genes & development·2026
Same journal

Mechanisms coordinating exit from the stem cell state in mammals.

Genes & development·2026
Same journal

Evolutionarily conserved spliceosome-exosome pathway in nuclear mRNA surveillance.

Genes & development·2026
Same journal

CDK1 and CEP97 cooperatively control centriole length to orchestrate ciliogenesis and developmental patterning.

Genes & development·2026
Same journal

Coupling of translesion synthesis with the replisome stabilized at stalled replication forks by ATR.

Genes & development·2026
See all related articles

Related Experiment Video

Updated: Apr 7, 2026

RNA Secondary Structure Prediction Using High-throughput SHAPE
13:42

RNA Secondary Structure Prediction Using High-throughput SHAPE

Published on: May 31, 2013

32.5K

Chaperoning 5S RNA assembly.

Clément Madru1, Simon Lebaron2, Magali Blaud1

  • 1Laboratoire de Cristallographie et RMN Biologiques, UMR, CNRS 8015, Université Paris Descartes, Faculté de Pharmacie, Sorbonne Paris Cité, 75006 Paris, France;

Genes & Development
|July 11, 2015
PubMed
Summary
This summary is machine-generated.

The Rpf2-Rrs1 complex guides the 5S ribosomal RNA (rRNA) and its associated proteins into preribosomes. This complex ensures proper assembly of the large ribosomal subunit by interacting with key ribosomal components.

Keywords:
5S RNPBrix domainp53ribosome assembly

More Related Videos

Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection
07:35

Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection

Published on: August 6, 2019

6.6K
Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
10:59

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Published on: May 13, 2019

10.3K

Related Experiment Videos

Last Updated: Apr 7, 2026

RNA Secondary Structure Prediction Using High-throughput SHAPE
13:42

RNA Secondary Structure Prediction Using High-throughput SHAPE

Published on: May 31, 2013

32.5K
Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection
07:35

Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection

Published on: August 6, 2019

6.6K
Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
10:59

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Published on: May 13, 2019

10.3K

Area of Science:

  • Molecular Biology
  • Structural Biology
  • Cell Biology

Background:

  • Eukaryotic ribosomes contain four ribosomal RNAs (rRNAs): 5.8S, 18S, 25S/28S, and 5S.
  • The 5S rRNA is transcribed separately and assembled into a ribonucleoprotein particle (RNP) before incorporation into preribosomes.
  • The Rpf2-Rrs1 complex (or its human homologues Bxdc1-hRrs1) is crucial for 5S RNP localization and assembly into preribosomes.

Purpose of the Study:

  • To structurally and functionally characterize the Rpf2-Rrs1 complex.
  • To understand the role of the Rpf2-Rrs1 complex in 5S RNP incorporation into preribosomes.
  • To elucidate the interactions of the Rpf2-Rrs1 complex within the pre-60S ribosome.

Main Methods:

  • Structural characterization of the Rpf2-Rrs1 complex.
  • Functional assays of the Rpf2-Rrs1 complex alone, with 5S RNA, and within pre-60S ribosomes.
  • Biochemical interaction studies.

Main Results:

  • The Rpf2-Rrs1 complex possesses a specific module for binding the 5S RNA E-loop.
  • The complex interacts with ribosomal protein Rpl5, ribosome assembly factor Rsa4, and the 25S rRNA.
  • Structural data reveals how the Rpf2-Rrs1 complex integrates the 5S RNP into the nascent large ribosomal subunit.

Conclusions:

  • The Rpf2-Rrs1 complex acts as a scaffold, mediating the precise incorporation of the 5S RNP into preribosomes.
  • This interaction network facilitates the initial conformation of the 5S RNP before its rotation in the mature large ribosomal subunit.
  • The findings provide insights into the intricate process of ribosome biogenesis and 5S rRNA maturation.