Jove
Visualize
Contact Us

Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

18.8K
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
18.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

VP2-targeted sandwich ELISA (sELISA) enables direct detection of Senecavirus A (SVA).

Journal of virology·2026
Same author

Immunoassay Detection of SARS-CoV-2 Using Monoclonal Antibody Binding to Viral Nucleocapsid Protein.

Microbial biotechnology·2025
Same author

Sensitive and selective detection of peanut allergen Ara h 1 by ELISA and lateral flow immunoassay.

Food chemistry·2022
Same author

A rapid and sensitive lateral flow immunoassay (LFIA) for the detection of gluten in foods.

Food chemistry·2021
Same author

Rapid Detection of Staphylococcal Enterotoxin-B by Lateral Flow Assay.

Monoclonal antibodies in immunodiagnosis and immunotherapy·2019
Same author

A Bioassay for Optimization of Macrophage-Conditioned Medium as a Culture Supplement to Promote Hybridoma Cell Survival and Growth.

Monoclonal antibodies in immunodiagnosis and immunotherapy·2018
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Apr 7, 2026

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay
06:15

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay

Published on: September 7, 2018

10.0K

Indirect ELISA.

Alice V Lin1

  • 1Produce Safety and Microbiology Unit (PSM), Western Regional Research Center (WRRC), Pacific West Area (PWA), Agricultural Research Service (ARS), United States Department of Agriculture (USDA), 800 Buchanan St., Albany, CA, 94710, USA, Alice.lin@ars.usda.gov.

Methods in Molecular Biology (Clifton, N.J.)
|July 11, 2015
PubMed
Summary
This summary is machine-generated.

This study details the indirect enzyme-linked immunosorbent assay (ELISA) for detecting antigens. It covers procedures and optimization methods for this sensitive immunoassay technique.

More Related Videos

High Throughput Sequential ELISA for Validation of Biomarkers of Acute Graft-Versus-Host Disease
09:00

High Throughput Sequential ELISA for Validation of Biomarkers of Acute Graft-Versus-Host Disease

Published on: October 31, 2012

16.4K
Author Spotlight: Expanding the Scope of Multiplex Immunoassays for Lyme Borreliosis Diagnostics and Pathogen Research
05:25

Author Spotlight: Expanding the Scope of Multiplex Immunoassays for Lyme Borreliosis Diagnostics and Pathogen Research

Published on: July 14, 2023

2.1K

Related Experiment Videos

Last Updated: Apr 7, 2026

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay
06:15

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay

Published on: September 7, 2018

10.0K
High Throughput Sequential ELISA for Validation of Biomarkers of Acute Graft-Versus-Host Disease
09:00

High Throughput Sequential ELISA for Validation of Biomarkers of Acute Graft-Versus-Host Disease

Published on: October 31, 2012

16.4K
Author Spotlight: Expanding the Scope of Multiplex Immunoassays for Lyme Borreliosis Diagnostics and Pathogen Research
05:25

Author Spotlight: Expanding the Scope of Multiplex Immunoassays for Lyme Borreliosis Diagnostics and Pathogen Research

Published on: July 14, 2023

2.1K

Area of Science:

  • Biochemistry
  • Immunology
  • Analytical Chemistry

Background:

  • The enzyme-linked immunosorbent assay (ELISA) is a highly sensitive immunoassay.
  • ELISA is valuable in research, diagnostics, and industrial quality control.
  • It detects antigens or antibodies bound to a solid surface using enzyme-linked antibodies and a substrate.

Purpose of the Study:

  • To provide detailed procedures for performing indirect ELISA.
  • To describe methods for optimizing indirect ELISA for polystyrene-immobilized antigens.
  • To lay the groundwork for assay automation and multiplexing.

Main Methods:

  • The study focuses on the indirect ELISA technique.
  • It involves detecting polystyrene-immobilized antigens.
  • Procedures for assay optimization are discussed.

Main Results:

  • The chapter outlines the step-by-step process for indirect ELISA.
  • It details how to optimize the assay for improved performance.
  • The described methods are applicable to antigen detection.

Conclusions:

  • Indirect ELISA is a versatile method for antigen detection.
  • Optimization is key for advancing ELISA towards automation and multiplexing.
  • This technique holds significant commercial and diagnostic value.