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Western Blotting: Sample Preparation to Detection
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Published on: October 14, 2010

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The Western Blot

Thomas S Hnasko1, Robert M Hnasko

  • 1Department of Neurosciences, School of Medicine, University of California San Diego (UCSD), 9500 Gilman Drive (BRF2), La Jolla, CA, 92093, USA, thnasko@ucsd.edu.

Methods in Molecular Biology (Clifton, N.J.)
|July 11, 2015
PubMed

Western blotting is a technique that involves the separation of proteins by gel electrophoresis, their blotting or transfer to a membrane, and selective immunodetection of an immobilized antigen. This is an important and routine method for protein analysis that depends on the specificity of antibody-antigen interaction and is useful for the qualitative or semiquantitative identification of specific proteins and their molecular weight from a complex mixture. This chapter will outline the requisite steps including gel electrophoresis of a protein sample, transfer of protein from a gel to a membrane support, and immunodetection of a target antigen.

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