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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
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Related Experiment Video

Updated: Apr 7, 2026

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
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Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

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Lateral Flow Immunoassay.

Kathryn H Ching1

  • 1Crystal Bioscience, 5980 Horton Street, Suite 405, Emeryville, CA, 94608, USA, ching.kathryn@gmail.com.

Methods in Molecular Biology (Clifton, N.J.)
|July 11, 2015
PubMed
Summary

Lateral flow immunoassays (LFIA) offer rapid, on-site diagnostic results without special equipment. This guide details designing and building LFIA prototypes for commercial use, leveraging accessible monoclonal antibody technology.

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Immunotechnology

Background:

  • Lateral flow immunoassays (LFIA) are widely used for rapid diagnostics due to their ease of use and quick results.
  • Their application spans home testing, emergency response, and food safety analysis.
  • LFIA performance critically depends on high-quality monoclonal antibodies for analyte detection.

Purpose of the Study:

  • To provide foundational knowledge for designing and constructing Lateral Flow Immunoassay (LFIA) prototypes.
  • To address the growing interest in developing LFIA prototypes for commercial manufacturing.
  • To guide smaller laboratories in leveraging accessible monoclonal antibody technology for LFIA development.

Main Methods:

  • Describing the fundamental principles of LFIA design.

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  • Outlining the essential steps for building an LFIA prototype.
  • Highlighting the role of monoclonal antibodies in LFIA functionality.

    • Main Results:

    • The chapter provides a comprehensive overview of LFIA prototype development.
    • It emphasizes the practical aspects of creating a functional diagnostic device.
    • The content aims to facilitate the transition from laboratory development to potential commercialization.

    Conclusions:

    • LFIA technology is becoming more accessible, enabling wider adoption in diagnostics.
    • Understanding the basics of LFIA design is crucial for developing new diagnostic tools.
    • This guide serves as a starting point for researchers and developers interested in LFIA prototyping.