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A high yield method for the isolation of sheep's liver cathepsin L
1Department of Biochemistry, University of Natal, Pietermaritzburg, South Africa.
Preparative Biochemistry
|January 1, 1989
Abstract:
A method, giving twice the yield of the previous method, for the isolation of sheep's liver cathepsin L is described. The method uses three phase partitioning (TPP) in t-butanol/water/ammonium sulphate mixtures, followed by two chromatographic steps, at different pH values, in a single column of S-Sepharose.
