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Updated: Apr 7, 2026
06:10
Live Imaging of Mouse Secondary Palate Fusion
Published on: July 27, 2017
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Molecular Anatomy of Palate Development
Andrew S Potter1, S Steven Potter1
1Cincinnati Children's Medical Center, Division of Developmental Biology, 3333 Burnet Ave., Cincinnati, OH, 45229, United States of America.
Plos One
|July 14, 2015
Summary
Researchers mapped gene expression in the developing mouse palate using RNA-Seq. This molecular atlas reveals region-specific patterns crucial for understanding craniofacial development and palate formation.
Area of Science:
- Craniofacial development
- Molecular anatomy
- Gene expression analysis
Background:
- The NIH FACEBASE consortium aims to centralize resources for craniofacial researchers.
- Understanding the molecular basis of craniofacial development is essential.
Purpose of the Study:
- To create a molecular anatomy of craniofacial development.
- To define gene expression programs driving murine palate development.
Main Methods:
- Utilized laser capture microdissection and RNA-Sequencing (RNA-Seq).
- Analyzed the E14.5 murine palate, divided into anterior/posterior and oral/nasal compartments.
- Generated 25 RNA-Seq datasets for detailed gene expression profiling.
Main Results:
- Provided a comprehensive, region-specific gene expression profile of the palate.
- Identified expression patterns for transcription factors, growth factors, and receptors.
- Inferred potential paracrine interactions between adjacent palatal compartments.
Conclusions:
- Developed an RNA-Seq based atlas of gene expression patterns in palate development at microanatomic resolution.
- The FACEBASE resource facilitates discovery in craniofacial research.
- High concordance with existing data and validation through immunostaining support the findings.
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