Related Experiment Video
Updated: Apr 7, 2026

Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography
Published on: January 18, 2020
Characterization of Capsular Polysaccharides and Their Glycoconjugates by Hydrodynamic Methods
Stephen E Harding1, Ali Saber Abdelhameed, Richard B Gillis
1National Centre for Macromolecular Hydrodynamics, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, LE12 5RD, UK, steve.harding@nottingham.ac.uk.
Abstract:
Hydrodynamic methods are relevant for the characterization of carbohydrates such as capsular bacterial polysaccharides or glycoconjugates in solution. This chapter focuses on the following hydrodynamic methods: sedimentation velocity analytical ultracentrifugation (SV AUC), dynamic light scattering (DLS), sedimentation equilibrium analytical ultracentrifugation (SE AUC), size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS), and capillary viscometry-intrinsic viscosity measurement. The chapter highlights the general principle of these five methods, describes experimental details, and specifies advances in the last years.
More Related Videos
Related Concept Videos
Electrophoresis: Overview
There...
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Size-Exclusion Chromatography
Silica particles offer advantages such as rigidity,...
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
SDS-PAGE
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...

