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Related Experiment Video

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Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping
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Electron accommodation dynamics in the DNA base thymine.

Sarah B King1, Anne B Stephansen2, Yuki Yokoi3

  • 1Department of Chemistry, University of California, Berkeley, California 94720, USA.

The Journal of Chemical Physics
|July 17, 2015
PubMed
Summary
This summary is machine-generated.

Investigating electron attachment to thymine, this study reveals that dipole-bound anions convert to valence-bound species near the vertical detachment energy. This conversion pathway is not observed at higher excitation energies.

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Area of Science:

  • Physical Chemistry
  • Chemical Physics
  • Molecular Dynamics

Background:

  • Understanding electron attachment to DNA bases is crucial for comprehending DNA damage and repair mechanisms.
  • The formation of temporary negative ions (TNIs) is a key step in electron-molecule interactions.

Purpose of the Study:

  • To investigate the dynamics of electron attachment to the DNA base thymine.
  • To explore the roles of dipole-bound and valence-bound anions in thymine.
  • To determine the influence of excitation energy on these electron attachment pathways.

Main Methods:

  • Femtosecond time-resolved photoelectron imaging of the gas-phase iodide-thymine (I(-)T) complex.
  • Utilizing ultraviolet pump and near-infrared probe pulses for electron detachment and analysis.
  • Velocity-map imaging of resulting photoelectrons.

Main Results:

  • Observed both dipole-bound and valence-bound negative ions of thymine at excitation energies near the vertical detachment energy (VDE) of I(-)T.
  • A longer rise time for the valence-bound state suggests conversion from the dipole-bound state.
  • No evidence of dipole-bound anions at excitation energies significantly above the VDE.

Conclusions:

  • The dipole-bound anion of thymine may act as a transient intermediate or "doorway" to the valence-bound anion.
  • This conversion pathway is specific to excitation energies close to the VDE of the complex.
  • Electron attachment dynamics to thymine are energy-dependent, influencing the formation of different anionic states.