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Preparation of protein conjugatable aflatoxin oxime derivative from significantly reduced starting quantities
S D Holladay1, D F Brownie, W T Corbett
1Department of Anatomy, Physiological Sciences, and Radiology, North Carolina State University, College of Veterinary Medicine, Raleigh 27606.
Veterinary and Human Toxicology
|December 1, 1989
Abstract:
A modification of an existing technique for the preparation of aflatoxin B1-(0-carboxymethyl) oxime is presented which allows for a significantly reduced starting amount of aflatoxin. About 80% of the aflatoxin was converted to its oxime. The new technique will be particularly valuable when protein conjugatable oximes of more expensive aflatoxin metabolites, such as aflatoxin M1, are required for immunoassay production.