Bonding efficacy of etch-and-rinse adhesives after dentin biomodification using ethanol saturation and collagen cross-linker pretreatment
Related Experiment Video
Updated: Apr 7, 2026
Development of Amelogenin-chitosan Hydrogel for In Vitro Enamel Regrowth with a Dense Interface
Published on: July 10, 2014
Pallavi Sharma1, Rajni Nagpal1, Shashi Prabha Tyagi1
1Department of Conservative Dentistry and Endodontics, Kothiwal Dental College and Research Centre, Moradabad, Uttar Pradesh, India.
Aim:
To evaluate whether the application of two simplified etch-and-rinse adhesives to biomodified dentin using ethanol-wet bonding (EWB) and collagen cross-linker (CCL) pretreatment improves their sealing ability.
Materials And Methods:
In 176 extracted human molars, the pulp-chambers were deroofed, and teeth were sectioned horizontally. Samples were randomly divided into eight groups according to four bonding techniques using two simplified etch-and-rinse adhesives; Adper Single Bond 2 (ASB) and XP Bond (XPB). The bonding protocols included: (a) Water-wet bonding (WWB); (b) EWB; (c) WWB and CCL application; (d) EWB and CCL application. After composite resin restorations, dye leakage evaluation and scanning electron microscope analysis were done. Leakage scores were statistically analyzed using Kruskal-Wallis and Mann-Whitney U tests at a significance level of P < 0.05.
Result:
For both ASB and XPB adhesives, least dye leakage was observed in EWB groups (b and d) (P = 0.918 and P = 0.399 respectively) which showed no significant difference, while maximum leakage scores were seen in WWB groups (a and c). Regardless of CCL application and adhesives used, EWB technique depicted (P = 0.003 and P = 0.004) significantly greater sealing ability than WWB.
Conclusion:
Bonding of ASB and XPB using EWB significantly improved their sealing ability. Biomodification using CCL pretreatment had no significant effect on the sealing ability of adhesives bonded with either WWB or EWB.
