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Subcellular compartmentation of dolichol taken up by mouse leukemia cells
W Jankowski1, A Szkopińska, E Kula-Swiezewska
1Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland.
Abstract:
[1-3H]Dolichol was intraperitoneally incubated with mouse leukemia Lcb 35 cells. The radioactive lipid taken up by the cells was subsequently recovered in two fractions separated by sucrose density gradient centrifugation: a particular, most probably, lysosomal fraction and a cytoplasmic one. Thin-layer chromatography of radioactive lipids present in both fractions revealed that majority of dolichol was unchanged and only a small part esterified with fatty acids. Distribution of dolichol in various mouse organs was examined. Of all the organs examined, spleen accumulated the highest amount of radioactivity.