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Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds
Published on: February 14, 2018
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Development of a rapid, reliable and quantitative method--"SPOTi" for testing antifungal efficacy
Khalida Rizi1, Sudaxshina Murdan2, Cynthia A Danquah3
1Department of Pharmaceutics, UCL School of Pharmacy, 29-39 Brunswick Square, London WC1N 1AX, UK; Department of Biological Sciences, Institute of Structural and Molecular Biology, Birkbeck, University of London, Malet Street, London WC1E 7HX, UK.
Journal of Microbiological Methods
|July 18, 2015
Summary
A new spot culture growth inhibition assay (SPOTi) is now available for testing antifungal susceptibility in dermatophytes. This method offers a high-throughput alternative for screening new antifungal drugs.
Area of Science:
- Medical Mycology
- Antimicrobial Drug Discovery
Background:
- A standardized reference method for antimicrobial susceptibility testing (AST) of dermatophytes is currently unavailable.
- Existing methods like disc-diffusion and broth micro-dilution can be time-consuming or lack throughput for large-scale screening.
Purpose of the Study:
- To adapt and validate the solid agar-based spot culture growth inhibition assay (SPOTi) for dermatophyte susceptibility testing.
- To establish fungal-SPOTi as a reliable and high-throughput method for screening novel antifungal agents against dermatophytes.
Main Methods:
- Adaptation of the established anti-tubercular SPOTi assay for dermatophyte pathogens.
- Correlation of fungal-SPOTi results with the disc-diffusion method.
- Validation of the assay using mycelial plugs.
Main Results:
- The fungal-SPOTi assay demonstrated successful adaptation for dermatophyte susceptibility testing.
- The fungal-SPOTi assay showed good correlation with the disc-diffusion method.
- The assay was validated using mycelial plugs, confirming its reliability.
Conclusions:
- The fungal-SPOTi assay is a viable and validated method for dermatophyte antimicrobial susceptibility testing.
- This assay serves as a high-throughput alternative to current methods for screening novel antifungal compounds.
- The fungal-SPOTi assay has the potential to accelerate antifungal drug discovery efforts.
