Related Experiment Video
Updated: Apr 6, 2026

Isolation, Culture, and Characterization of Prostate Cancer-Associated Fibroblasts
Published on: August 1, 2025
Increased expression of ARF GTPases in prostate cancer tissue
Claire Morgan1, Paul D Lewis2, Lynda Hopkins3
1Institute of Life Science, Swansea University, Swansea, UK.
Purpose:
ARFs are a family of Ras-related GTP binding proteins, ARF6, in particular, is implicated in cancer invasion and metastasis. However, the role of ARF proteins in prostate cancer have yet to be investigated.
Methods:
Immunohistochemical staining for ARF6 was performed on a prostate cancer tissue microarray with patient matched normal specimens.
Results:
Antibody staining was significantly over-expressed in prostate cancer patient samples compared to normal patient tissue and a trend towards increased staining intensity in cancer samples with Gleason scores of 8 and above (metastatic disease).
Conclusion:
Due to high homology between members of the ARF family we could not determine if ARF 6 was the only ARF over-expressed in the prostate cancer samples. However, we are the first to show that ARF-GTPases are over expressed in prostate cancer which provides further insight into the molecular biology of prostate cancer.
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