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Updated: Apr 6, 2026

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Published on: November 18, 2022
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Substrate-constituted three-liquid-phase system: a green, highly efficient and recoverable platform for interfacial
Zhigang Li1, Huayong Chen, Weifei Wang
1School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China. yangbo@scut.edu.cn.
Summary
This study demonstrates highly efficient interfacial enzymatic hydrolysis of oil using a three-liquid-phase system. This innovative approach enhances enzyme activity and allows for easy product separation and enzyme reuse.
Area of Science:
- Biocatalysis
- Chemical Engineering
- Enzyme Technology
Background:
- Enzymatic hydrolysis of oils is crucial for various industrial applications.
- Traditional methods often face challenges with efficiency and product inhibition.
Purpose of the Study:
- To develop a highly efficient method for interfacial enzymatic hydrolysis of oil.
- To investigate the benefits of a three-liquid-phase system for enzymatic reactions.
Main Methods:
- Utilized a three-liquid-phase system where oil served as one phase.
- Employed enzymatic hydrolysis to break down oil at the interface.
- Analyzed factors contributing to catalytic efficiency and enzyme reusability.
Main Results:
- Achieved highly efficient interfacial enzymatic hydrolysis of oil.
- Observed enlarged interfacial area leading to increased catalytic efficiency.
- Demonstrated relief of product inhibition, enhancing enzyme performance.
- Showcased convenient product isolation and high enzyme reusability.
Conclusions:
- The three-liquid-phase system significantly enhances oil hydrolysis efficiency.
- This system offers practical advantages in product recovery and enzyme recycling.
- The findings present a promising strategy for industrial biocatalysis.

