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Related Concept Videos

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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CRISPR/Cas9 Genome Editing01:28

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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RNA Interference01:23

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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Putting Non-coding RNA on Display with CRISPR.

Pablo Perez-Pinera1, Matthew F Jones2, Ashish Lal2

  • 1Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.

Molecular Cell
|July 18, 2015
PubMed
Summary
This summary is machine-generated.

Researchers developed CRISPR Display (CRISP-Disp), a versatile platform for targeting non-coding RNAs (ncRNAs) to specific DNA locations. This tool aids synthetic biology and understanding ncRNA roles.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Synthetic Biology

Background:

  • Non-coding RNAs (ncRNAs) play crucial roles in gene regulation.
  • Understanding ncRNA function requires precise tools for genomic targeting.
  • Existing methods may lack flexibility or multiplexing capabilities.

Purpose of the Study:

  • To introduce CRISPR Display (CRISP-Disp), a novel platform for targeting ncRNAs.
  • To provide a flexible, modular, and multiplexable system for ncRNA research.
  • To facilitate synthetic biology applications and ncRNA functional studies.

Main Methods:

  • Development of the CRISPR Display (CRISP-Disp) platform.
  • Demonstration of its capability to target various ncRNAs to genomic loci.
  • Leveraging CRISPR technology for precise RNA-guided DNA targeting.

Main Results:

  • CRISP-Disp offers a sophisticated and adaptable approach for ncRNA manipulation.
  • The platform enables multiplexing, allowing simultaneous targeting of multiple ncRNAs.
  • Successful targeting of diverse ncRNAs to specific genomic locations was achieved.

Conclusions:

  • CRISP-Disp is a powerful new tool for advancing ncRNA research.
  • The platform significantly facilitates synthetic biology endeavors.
  • CRISP-Disp enables deeper elucidation of non-coding RNA functions within the genome.