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Related Experiment Video

Updated: Apr 6, 2026

Constructing Thioether/Vinyl Sulfide-tethered Helical Peptides Via Photo-induced Thiol-ene/yne Hydrothiolation
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A Thiol-Ene Coupling Approach to Native Peptide Stapling and Macrocyclization.

Yuanxiang Wang1, Danny Hung-Chieh Chou2

  • 1Department of Biochemistry, University of Utah, 15 N. Medical Drive East 4100, Salt Lake City, UT 84112 (USA).

Angewandte Chemie (International Ed. in English)
|July 21, 2015
PubMed
Summary
This summary is machine-generated.

We developed a novel peptide stapling method using thiol-ene chemistry for efficient macrocyclization. This technique stabilizes peptide structures and enables targeted drug development for cancer therapies.

Keywords:
bioconjugationpeptide macrocyclizationpeptide staplingthiol-ene coupling

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Area of Science:

  • Medicinal Chemistry
  • Organic Chemistry
  • Biochemistry

Background:

  • Peptide-based therapeutics offer high specificity but often suffer from poor stability and bioavailability.
  • Stabilizing peptide secondary structures, such as alpha-helices, is crucial for enhancing their therapeutic potential.
  • Developing efficient and versatile methods for peptide modification is essential for drug discovery.

Purpose of the Study:

  • To introduce a novel and robust peptide stapling and macrocyclization method.
  • To demonstrate the utility of this method for helix stabilization and the development of bioactive peptide analogues.
  • To explore the application of this method in creating peptide-based cancer therapeutics.

Main Methods:

  • Utilized thiol-ene click chemistry between cysteine residues and α,ω-dienes for peptide macrocyclization.
  • Developed a method for selective modification of native, unprotected peptides.
  • Synthesized stapled Axin and p53 mimetic analogues with hydrocarbon linkers.

Main Results:

  • Achieved high yields in peptide stapling and macrocyclization reactions.
  • Demonstrated increased alpha-helicity in stapled Axin mimetic analogues.
  • Showcased stapled p53 mimetic analogues' ability to inhibit the p53-MDM2 interaction and selectively kill p53 wild-type cancer cells.

Conclusions:

  • Established a versatile and robust peptide stapling methodology applicable to both synthetic and expressed peptides.
  • Validated the potential of this method for developing conformationally stabilized peptides with therapeutic applications.
  • Highlighted the promise of this approach for creating novel peptide drugs, particularly in oncology.