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Related Experiment Video

Updated: Apr 6, 2026

Author Spotlight: Advancing Protein Glycosylation Research Using a Fully Automated System

Author Spotlight: Advancing Protein Glycosylation Research Using a Fully Automated System

Published on: June 28, 2024

Automated, high-throughput serum glycoprofiling platform.

H Stöckmann¹, R O'Flaherty, B Adamczyk

¹NIBRT GlycoScience Group, NIBRT - The National Institute for Bioprocessing Research and Training, Fosters Avenue, Mount Merrion, Blackrock, Co.Dublin, Ireland. pauline.rudd@nibrt.ie.

Integrative Biology : Quantitative Biosciences From Nano to Macro

|July 21, 2015

Summary

This summary is machine-generated.

Automated N-glycan sample preparation accelerates biomarker discovery. This high-throughput platform enables rapid glycoprofiling of serum samples, facilitating the identification of clinical glycobiomarkers.

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Area of Science:

Glycomics
Biomarker Discovery
Analytical Chemistry

Background:

Complex carbohydrates (glycans) show high sensitivity to pathological changes, making them promising biomarker candidates.
Clinical glycobiomarker discovery is hindered by the lack of high-throughput glycoanalytical workflows for large sample sets.

Purpose of the Study:

To develop a robotized platform for rapid, high-throughput serum-based N-glycan sample preparation.
To enable quantitative glycomics data generation for efficient biomarker discovery.

Main Methods:

Automated glycoprotein denaturation, enzymatic N-glycan release, and solid-supported hydrazide purification.
Fluorescent labeling of glycans for accurate quantitation using ultra-high performance liquid chromatography (UPLC).
Automation on an eight-channel liquid handling workstation for processing nearly 100 samples in 14 hours.

Main Results:

The developed platform provides a fully automated workflow for serum N-glycan sample preparation.
Excellent reproducibility was achieved in sample preparation.
The workflow significantly enhances the speed of glycoprofiling for large clinical sample sets.

Conclusions:

The robotized platform greatly facilitates serum-based glycobiomarker discovery.
High-throughput glycoprofiling is now feasible for large-scale clinical studies.
This advancement addresses the bottleneck in identifying novel clinical glycobiomarkers.