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Updated: Apr 6, 2026
Isolation of Mouse Lung Dendritic Cells
Published on: November 22, 2011
Resveratrol inhibits dysfunction of dendritic cells from chronic obstructive pulmonary disease patients through
Xudong Wang1, Chao Zhang1, Guangsu Huang1
1Department of Critical Care Medicine, The Second Affiliated Hospital of Xuzhou Medical College Jiangsu 221006, PR China.
Background:
Resveratrol has demonstrated many beneficial effects against aging, including anti-inflammatory and antioxidant roles. The present study was designed to observe the effects of resveratrol on the dysfunction of dendritic cells (DCs) from COPD patients and its possible mechanism and use in the treatment for COPD.
Methods:
Flow cytometry analysis was used to examine the expression of costimulatory markers CD80 and CD86 and ELISA was used to examine the secretion of IFN-α. Expression of miR-34 was examined by using real-time PCR. Expression vector of miR-34, LV3-miR-34 was also constructed and transfected into DCs to observe the effects on functions of DCs.
Results:
The results showed that there was remarkable upregulation of CD80 and CD86 and secretion of cytokines IFN-α in DCs from COPD patients. Resveratrol displayed a dose-dependent cytotoxicity action over 10 µg/mL and pretreatment with resveratrol inhibited upregulation of CD80 and CD86 and secretion of cytokines IFN-α. Further study showed resveratrol upregulated the expression of miR-34, which inhibited the dysfunction of DCs.
Conclusion:
These proofs suggest that resveratrol inhibited dysfunction of DCs from COPD patients through promoting miR-34.
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