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Updated: Apr 6, 2026
From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
Published on: October 9, 2014
Inferring transient particle transport dynamics in live cells
Nilah Monnier1, Zachary Barry1, Hye Yoon Park2,3,4
1Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
Abstract:
Live-cell imaging and particle tracking provide rich information on mechanisms of intracellular transport. However, trajectory analysis procedures to infer complex transport dynamics involving stochastic switching between active transport and diffusive motion are lacking. We applied Bayesian model selection to hidden Markov modeling to infer transient transport states from trajectories of mRNA-protein complexes in live mouse hippocampal neurons and metaphase kinetochores in dividing human cells. The software is available at http://hmm-bayes.org/.
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