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Development of enterobacterium-specific oligonucleotide probes based on the surface-exposed regions of outer membrane
G Spierings1, H Hofstra, J Huis in'T Veld
1Institute of Molecular Biology and Medical Biotechnology, State University of Utrecht, The Netherlands.
Applied and Environmental Microbiology
|December 1, 1989
Abstract:
Outer membrane proteins of members of the family Enterobacteriaceae consist of conserved membrane-spanning segments and hypervariable, surface-exposed regions. We demonstrate that the hypervariable DNA segments corresponding to the surface-exposed regions of these proteins can be used to develop specific DNA probes for the identification of members of the family Enterobacteriaceae.