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Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Related Experiment Video

Updated: Apr 6, 2026

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Published on: July 10, 2016

Large-Volume Microfluidic Cell Sorting for Biomedical Applications.

Majid Ebrahimi Warkiani^1,2, Lidan Wu³, Andy Kah Ping Tay¹

¹BioSystems and Micromechanics IRG, Singapore-MIT Alliance for Research and Technology (SMART) Centre, Singapore 138602;

Annual Review of Biomedical Engineering

|July 22, 2015

Summary

This summary is machine-generated.

High-throughput microfluidic cell sorting overcomes previous limitations, enabling new diagnostic and therapeutic applications. This review highlights advancements in large-volume microfluidic methods and their innovative uses.

Keywords:

blood transfusion cell sorting circulating cancer cells high-throughput inertial large-volume microfluidic separation sepsis

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Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology

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Area of Science:

Biotechnology
Medical Devices
Cell Biology

Background:

Microfluidic cell-separation technologies have been researched for nearly 20 years.
Limited throughput has historically restricted the application scope of microfluidic cell separation.

Purpose of the Study:

To review recent progress in engineering large-volume microfluidic cell-sorting methods.
To explore novel diagnostic and therapeutic applications enabled by high-throughput microfluidics.

Main Methods:

Focus on advancements in microfluidic engineering for cell sorting.
Analysis of methods enabling large-volume processing.

Main Results:

Recent microfluidic advances facilitate high-throughput cell sorting and separation.
These advancements unlock previously unfeasible diagnostic and therapeutic applications.

Conclusions:

High-throughput microfluidic cell sorting significantly expands the potential of microfluidic technologies.
Engineering large-volume microfluidic systems is key to realizing new applications in diagnostics and therapeutics.