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Updated: Aug 14, 2026
Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation
Published on: October 20, 2014
A study of human embryonic cells treated with UV-inactivated measles virus
N Mazur1, M Korbecki, R Zahorska
1Serum and Vaccine Research Laboratory, Warsaw.
Abstract:
Some features of persistent infection were observed in human embryonic fibroblast cells (CLV21K cells) after single treatment with UV-inactivated measles virus. Morphological changes and chromosomal abnormalities were found. In subsequent passages of CLV21K cells, viral antigens were discovered by rosette haemadsorption, immunofluorescence tests and electron microscope or scanning electron microscope studies. No infectious virus was found.
Insights
UV-inactivated measles virus induced persistent infection in human embryonic fibroblast cells, causing cell abnormalities. Viral antigens were detected, but no infectious virus was found in later cell passages.
Area of Science:
- Virology
- Cell Biology
- Genetics
Background:
- Persistent viral infections can alter host cell characteristics.
- Measles virus (MeV) is a significant human pathogen.
- UnderstandingMeV-host interactions is crucial for disease management.
Purpose of the Study:
- To investigate the effects of UV-inactivated measles virus on human embryonic fibroblast cells.
- To characterize the nature of persistent infection induced by the virus.
Main Methods:
- Treatment of CLV21K cells with UV-inactivated measles virus.
- Morphological and chromosomal abnormality analysis.
- Detection of viral antigens using rosette haemadsorption, immunofluorescence, and electron microscopy.
Main Results:
- Observed features of persistent infection in CLV21K cells.
- Identified morphological changes and chromosomal abnormalities.
- Detected viral antigens in subsequent cell passages, but no infectious virus.
Conclusions:
- UV-inactivated measles virus can establish a non-productive persistent infection in human embryonic fibroblast cells.
- This persistent infection is characterized by cellular alterations and the presence of viral antigens.
- The findings suggest a potential role for non-infectious viral components in cellular modulation.
