[Luminescence of activated phagocytes]

By means of microscope-cytofluorometry "Luman-13" intensive proper luminescence of neutrophilic granulocytes and monocytes in blood and pus, that reduce nitroblue tetrazolium has been investigated. The luminescence is localized in the cytoplasmic areas free from formazan; formazan itself does not demonstrate any luminescence. Two types of luminescence have been revealed: dark blue-blue and yellow-green. According to the luminescence spectral analysis stimulation with radiation at the wave length 360 nm produces luminescence of the I type with its maximum in the area of 450-470 nm and 520-535 nm. When stimulation with light at the wave length is 460 nm, the luminescence spectrum++ of the II type possesses the main maximum at 535 nm. Luminescence spectra of neutrophilic granulocytes and monocytes are similar. The luminescence stripe in the area 450-470 nm results from reduced pyridinenucleotides, luminescence with the wave length 520-535 nm--from oxidized flavoproteins.