Glucosinolate Diversity in Bretschneidera sinensis of Chinese Origin

The glucosinolate (GL) profile in several plant parts (leaf, branch, bark, root, and fruit) of Bretschneidera sinensis from three geographical regions of the People's Republic of China was established for the first time by HPLC. During this investigation, benzyl GL (1), 4-hydroxybenzyl GL (2), 2-hydroxy-2-methylpropyl GL (3), and 4-methoxybenzyl GL (4) were identified. In addition, one new GL, 3-hydroxy-4-methoxybenzyl GL (5), was isolated in a minor amount from the fruit and characterized by spectroscopic data interpretation. Furthermore, traces of 4-hydroxy-3-methoxyphenylacetonitrile were detected by GC-MS analysis in the fruits, thus confirming the presence of the regioisomeric 4-hydroxy-3-methoxybenzyl GL (6). GLs 1-5 were also quantified for the first time by HPLC in the various plant organs.