Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Attachment of Sister Chromatids02:57

Attachment of Sister Chromatids

4.2K
As cells progress into mitosis, the nuclear envelope breaks down, and the condensed chromosomes are exposed to the array of bipolar microtubules of the mitotic spindle. The kinetochore, a large, disc-shaped protein complex, is present at the centromere region of the sister chromatids and acts as a binding site for the microtubules.  Usually, the plus-end of a single microtubule is embedded within the kinetochore. However, some kinetochores first establish lateral contact with the side-wall...
4.2K
Attachment of Sister Chromatids02:57

Attachment of Sister Chromatids

1.9K
1.9K
Forces Acting on Chromosomes02:11

Forces Acting on Chromosomes

4.1K
During mitosis, chromosome movements occur through the interplay of multiple piconewton level forces. In prometaphase, these forces help in chromosome assembly or congression at the equatorial plane, eventually leading to their alignment at the metaphase plate. The forces acting on the chromosomes are space and time-dependent; therefore, they vary with the position of the chromosomes as the cell progresses through mitosis. 
Microtubules and motor proteins exert two types of forces on...
4.1K
Forces Acting on Chromosomes02:11

Forces Acting on Chromosomes

2.0K
2.0K
The Mitotic Spindle02:27

The Mitotic Spindle

8.4K
The mitotic spindle—or spindle apparatus—is a eukaryotic, cytoskeletal structure made up of long protein fibers called microtubules. Formed during cell division, the spindle separates sister chromatids and moves them to opposite ends of a parental cell, where the now individual chromosomes are distributed to two daughter cell nuclei.
The bipolar configuration of the mitotic spindle facilitates chromosomal segregation, preparing the cell for division. One mechanism that ensures...
8.4K
The Mitotic Spindle02:27

The Mitotic Spindle

5.2K
5.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Affinage: genome-scale mechanistic gene annotation from the published literature.

ArXiv·2026
Same author

A one-week automated genome-wide optical pooled screen using OttoSeq.

Genome biology·2026
Same author

The one-week automated genome-wide optical pooled screen.

bioRxiv : the preprint server for biology·2026
Same author

5' UTR length shapes alternative N-terminal protein isoforms across cancers and in rare disease.

EMBO reports·2026
Same author

The dynamics of centromere assembly and disassembly during quiescence.

The Journal of cell biology·2026
Same author

Global stabilization of the transcriptome in mitotic cells.

The EMBO journal·2026

Related Experiment Video

Updated: Apr 6, 2026

Live Cell Imaging of Chromosome Segregation During Mitosis
06:39

Live Cell Imaging of Chromosome Segregation During Mitosis

Published on: March 14, 2018

10.0K

Chromosome Segregation: A Spatial Code to Correct Kinetochore-Microtubule Attachments.

Julie K Monda1, Iain M Cheeseman1

  • 1Whitehead Institute for Biomedical Research, Cambridge, MA, USA; Department of Biology, MIT, Nine Cambridge Center, Cambridge, MA 02142, USA.

Current Biology : CB
|July 22, 2015
PubMed
Summary

Accurate chromosome segregation relies on correcting errors in kinetochore-microtubule attachments before cell division. New research reveals a mechanism involving Aurora A kinase that uses chromosome position to fix these errors.

More Related Videos

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
05:35

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

15.8K
Using Mouse Oocytes to Assess Human Gene Function During Meiosis I
11:13

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

Published on: April 10, 2018

9.6K

Related Experiment Videos

Last Updated: Apr 6, 2026

Live Cell Imaging of Chromosome Segregation During Mitosis
06:39

Live Cell Imaging of Chromosome Segregation During Mitosis

Published on: March 14, 2018

10.0K
Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
05:35

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

15.8K
Using Mouse Oocytes to Assess Human Gene Function During Meiosis I
11:13

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

Published on: April 10, 2018

9.6K

Area of Science:

  • Cell Biology
  • Genetics
  • Molecular Biology

Background:

  • Accurate chromosome segregation is crucial for cell division, preventing aneuploidy.
  • Kinetochore-microtubule (KT-MT) attachments must be corrected before anaphase onset.
  • Errors in KT-MT attachments can lead to chromosome mis-segregation and developmental abnormalities.

Purpose of the Study:

  • To elucidate the mechanism of error correction for erroneous kinetochore-microtubule interactions.
  • To investigate the role of Aurora A kinase in spatial error detection.
  • To understand how chromosome positioning influences the correction of KT-MT attachments.

Main Methods:

  • Live-cell imaging to observe chromosome dynamics and KT-MT attachments.
  • Depletion or inhibition of Aurora A kinase to assess its role.
  • Analysis of chromosome positioning within the mitotic spindle.

Main Results:

  • Aurora A kinase is recruited to kinetochores based on chromosome position.
  • This recruitment facilitates the correction of erroneous KT-MT attachments.
  • Spatial positioning acts as a key signal for error correction, mediated by Aurora A.

Conclusions:

  • Aurora A-mediated error correction is a critical checkpoint for ensuring accurate chromosome segregation.
  • Chromosome spatial positioning within the spindle is a key determinant for activating this error correction pathway.
  • These findings provide new insights into the molecular mechanisms preventing aneuploidy during cell division.