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Designing Automated, High-throughput, Continuous Cell Growth Experiments Using eVOLVER

Designing Automated, High-throughput, Continuous Cell Growth Experiments Using eVOLVER

Published on: May 19, 2019

A Small-Volume, Low-Cost, and Versatile Continuous Culture Device.

Dominick Matteau¹, Vincent Baby¹, Stéphane Pelletier²

¹Département de biologie, Université de Sherbrooke, Sherbrooke, Québec, Canada.

|July 22, 2015

Summary

Researchers developed an affordable, customizable continuous culture system for microbial ecology and synthetic biology. This versatile device offers flexibility for various experimental needs, enabling precise control over cell population growth conditions.

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Area of Science:

Biotechnology
Microbiology
Synthetic Biology

Background:

Commercial continuous culture instruments are often expensive and lack flexibility for small-volume, milliliter-range experiments.
Existing systems may not meet the diverse needs of various laboratory settings.

Purpose of the Study:

To develop a versatile, affordable, and customizable continuous culture system.
To provide detailed instructions and software for users to assemble and operate their own instrument.

Main Methods:

Designed a system with three simultaneously controllable culture chambers using readily available components.
Enabled programming for turbidostat or chemostat modes with real-time feedback or calibrated medium delivery.
Integrated phenol red detection for monitoring metabolic activity via pH changes, offering an alternative to turbidity measurements.

Main Results:

The developed system is affordable and customizable, allowing for simultaneous control of three culture chambers.
The device can operate as either a turbidostat or chemostat, with flexible medium delivery options.
Phenol red can be used as a pH indicator to monitor metabolic activity, providing an alternative to turbidity.

Conclusions:

This continuous culture system offers a flexible and cost-effective solution for researchers.
It is applicable to diverse fields including microbial ecology, systems biology, and synthetic biology.
The system empowers laboratories with a customizable tool for long-term cell culture maintenance.