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Updated: Aug 19, 2026

An Optimized Protocol to Analyze Glycolysis and Mitochondrial Respiration in Lymphocytes
Published on: November 21, 2016
Energy state, glycolytic intermediates and mitochondrial function in the liver during reversible and irreversible
S Kopprasch1, U Hörkner, H Orlik
1Medical Academy of Dresden, Institute of Pathological Biochemistry, GDR.
Abstract:
Metabolites of the energy and carbohydrate metabolism and mitochondrial function in the liver were compared in rats with reversible as well as with irreversible shock. 6 h after induction of shock there was a close correlation between the severity of shock and the energy state of the liver. Only rats with irreversible shock showed a marked deterioration in parameters of the adenylate system, whereas in animals with reversible shock the energy state remained at control levels. Liver glycogen and glucose stores declined similarly in all shocked rats. The capacity of isolated liver mitochondria to produce ATP did not essentially differ in reversible and irreversible shock. Further investigations should consider the intracellular environment in evaluating the mitochondrial function in vivo during endotoxin shock.
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