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Correlation between polarization sensitive optical coherence tomography and second harmonic generation microscopy in

Viet-Hoan Le1, Seunghun Lee2, Bumju Kim2

  • 1Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, 77 Cheongam-ro, Nam-gu, Pohang, Gyeongbuk 790-784, South Korea.

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|July 24, 2015
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Summary

Polarization sensitive optical coherence tomography (PS-OCT) and second harmonic generation (SHG) microscopy reveal a direct correlation between bulk skin birefringence and collagen fiber orientation. This relationship aids in understanding skin pathologies.

Keywords:
(170.2655) Functional monitoring and imaging(170.3890) Medical optics instrumentation(170.4500) Optical coherence tomography(180.4315) Nonlinear microscopy(260.1440) Birefringence

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Area of Science:

  • Biomedical Optics
  • Dermatology
  • Materials Science

Background:

  • Polarization sensitive optical coherence tomography (PS-OCT) and second harmonic generation (SHG) microscopy are advanced 3D optical imaging techniques.
  • Both modalities provide insights into skin collagen, a key structural protein.
  • Previous studies applied PS-OCT and SHG microscopy to skin pathologies independently, but their interrelationship remained unexplored.

Purpose of the Study:

  • To investigate the relationship between PS-OCT-derived birefringence and SHG microscopy-derived collagen orientation in skin.
  • To quantitatively assess the correlation between bulk tissue properties and microscopic collagen fiber organization.
  • To evaluate these parameters in normal and burn-damaged rat skin.

Main Methods:

  • Ex vivo imaging of normal and burn-damaged rat skin samples using both PS-OCT and SHG microscopy.
  • Quantitative analysis of PS-OCT images to determine tissue birefringence.
  • Quantitative analysis of SHG images using 2D Fourier transform to calculate collagen orientation indices.

Main Results:

  • A significant linear correlation was observed between tissue birefringence measured by PS-OCT and collagen orientation indices from SHG microscopy.
  • Higher birefringence values corresponded to higher collagen orientation indices.
  • Burn-damaged skin exhibited reduced birefringence and collagen orientation compared to normal skin.

Conclusions:

  • The study establishes a quantitative relationship between macroscopic skin birefringence and microscopic collagen fiber arrangement.
  • This correlation provides a valuable link between bulk tissue properties and structural collagen organization.
  • The findings suggest potential applications for combined PS-OCT and SHG imaging in diagnosing and monitoring skin conditions characterized by collagen alterations.