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In Vivo Monitoring of Transcriptional Activity During Metabolic Transition Using a Bioluminescent Reporter in Yeast
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Glucose repression in Saccharomyces cerevisiae
1Department of Biology and Biological Engineering, Kemivägen 10, Chalmers University of Technology, SE41296 Gothenburg, Sweden Novo Nordisk Foundation Center for Biosustainability, Chalmers University of Technology, SE41296 Gothenburg, Sweden.
FEMS Yeast Research
|July 25, 2015
Summary
Glucose strongly influences yeast metabolism, suppressing alternative fuel use and respiration. This review details glucose repression mechanisms, focusing on the Snf3/Rgt2 and Snf1 pathways in Saccharomyces cerevisiae.
Area of Science:
- Microbiology
- Molecular Biology
- Biochemistry
Background:
- Glucose is the preferred energy source for Saccharomyces cerevisiae.
- Glucose presence represses the utilization of alternative carbon sources, respiration, and gluconeogenesis.
- This glucose effect involves complex signaling and metabolic interactions regulating gene expression.
Purpose of the Study:
- To review the effects of glucose repression on yeast carbon metabolism.
- To highlight the roles of the Snf3/Rgt2 glucose-sensing pathway.
- To explain the involvement of Snf1 signal transduction in glucose repression.
Main Methods:
- Literature review of studies on yeast carbon metabolism.
- Analysis of signaling pathways involved in glucose sensing.
- Examination of transcriptional, post-transcriptional, and post-translational regulation.
Main Results:
- Glucose repression is a dominant metabolic control mechanism in yeast.
- The Snf3/Rgt2 pathway is crucial for sensing external glucose levels.
- Snf1 signal transduction plays a key role in managing glucose repression.
Conclusions:
- Understanding glucose repression is vital for yeast metabolic engineering.
- The Snf3/Rgt2 and Snf1 pathways are central regulators of yeast carbon metabolism.
- Glucose's impact extends beyond transcriptional control, affecting multiple regulatory levels.
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