Related Experiment Videos

Haemolysins of Vibrio parahaemolyticus strains passaged in mice

I Karunasagar, I Karunasagar, G R Malathi

    The Indian Journal of Medical Research
    |November 1, 1989
    PubMed

    Insights

    Phenotypically non-haemolytic Vibrio parahaemolyticus strains can produce heat-stable and heat-labile haemolysins after passage in mice. The induced heat-stable haemolysin is immunologically distinct from the Kanagawa positive strain haemolysin.

    Area of Science:

    • Microbiology
    • Bacterial Pathogenesis

    Background:

    • Vibrio parahaemolyticus is a significant foodborne pathogen.
    • Kanagawa-positive strains typically produce thermostable direct haemolysin (TDH), a virulence factor.
    • Non-haemolytic strains present a diagnostic challenge.

    Purpose of the Study:

    • To investigate the haemolytic potential of phenotypically non-haemolytic V. parahaemolyticus strains.
    • To characterize the induced haemolysins in terms of heat stability and immunological properties.

    Main Methods:

    • Passaging non-haemolytic V. parahaemolyticus strains in mice.
    • Induction and collection of haemolysins.
    • Testing haemolysin activity (heat stability).
    • Immunological comparison of induced haemolysins with TDH.

    Main Results:

    • Non-haemolytic V. parahaemolyticus strains induced haemolysin production after mouse passage.
    • Both heat-stable and heat-labile haemolysins were observed, varying by strain.
    • The induced heat-stable haemolysin showed immunological differences compared to TDH from Kanagawa-positive strains.

    Conclusions:

    • In vivo passage can alter the virulence factor expression of V. parahaemolyticus.
    • Non-haemolytic strains may possess cryptic haemolytic potential.
    • The induced haemolysins may represent novel virulence factors or variations.

    Related Concept Videos